Mouse Recombinant Monoclonal VL1 antibody. Suitable for I-ELISA and reacts with Recombinant full length protein - Human papillomavirus type 18 samples.
Images
![Indirect ELISA - Anti-Human Papilloma Virus type 18 L1 protein antibody [SK18L1_19H3] (AB323836), expandable thumbnail](https://content.abcam.com/products/images/human-papilloma-virus-type-18-l1-protein-antibody-sk18l119h3-ab323836--indirect-elisa-img446006.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Human Papilloma Virus type 18 L1 protein antibody [SK18L1_19H3] (AB323836)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | ICC/IF | |
|---|---|---|
| Human papillomavirus type 18 | Predicted | Not recommended |
| Recombinant full length protein - Human papillomavirus type 18 | Tested | Not recommended |
| Transfected cell line - Human papillomavirus type 18 | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human papillomavirus type 18 | Dilution info 62.5 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus type 18 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human papillomavirus type 18 | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human papillomavirus type 18, Human papillomavirus type 18, Recombinant full length protein - Human papillomavirus type 18 | Dilution info - | Notes - |
Target data
Function
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
Alternative names
Major capsid protein L1, L1
Mouse Recombinant Monoclonal VL1 antibody. Suitable for I-ELISA and reacts with Recombinant full length protein - Human papillomavirus type 18 samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SK18L1_19H3
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody has been tested with recombinant protein and do not show cross-react with L1 protein from HPV type 45, HPV type 35, HPV type 31, HPV type 16 and HPV type 6 .
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Indirect ELISA - Anti-Human Papilloma Virus type 18 L1 protein antibody [SK18L1_19H3] (ab323836), expandable thumbnail](https://content.abcam.com/products/images/human-papilloma-virus-type-18-l1-protein-antibody-sk18l119h3-ab323836--indirect-elisa-img446006.jpg "Indirect ELISA - Anti-Human Papilloma Virus type 18 L1 protein antibody [SK18L1_19H3] (ab323836)")
Indirect ELISA - Anti-Human Papilloma Virus type 18 L1 protein antibody [SK18L1_19H3] (ab323836)
Indirect ELISA analysis of ab323836 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at 1:1000 dilution dilution.
Antigen: HPV 18 L1 protein, HPV 45 L1 protein, HPV 31 L1 protein, HPV 35 L1 protein, HPV 16 L1 protein, HPV 6 L1 protein.
Antigen concentration: 5000 ng/ml
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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