Mouse Recombinant Monoclonal VL1 antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - Human papillomavirus type 6a, Recombinant fragment - Human papillomavirus type 6a samples.
Images
![Indirect ELISA - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (AB322925), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-6-l1-protein-antibody-lh6l1d-ab322925--indirect-elisa-img441950.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (AB322925)")
![Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (AB322925), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-6-l1-protein-antibody-lh6l1d-ab322925--immunocytochemistry-immunofluorescence-img441928.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (AB322925)")
![Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (AB322925), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-6-l1-protein-antibody-lh6l1d-ab322925--immunocytochemistry-immunofluorescence-img441927.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (AB322925)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | I-ELISA | |
|---|---|---|
| Human papillomavirus type 6a | Predicted | Predicted |
| Recombinant fragment - Human papillomavirus type 6a | Not recommended | Tested |
| Transfected cell line - Human papillomavirus type 6a | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human papillomavirus type 6a | Dilution info 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus type 6a | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human papillomavirus type 6a | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human papillomavirus type 6a | Dilution info 31.25 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus type 6a | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human papillomavirus type 6a | Dilution info - | Notes - |
Target data
Function
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
Alternative names
Major capsid protein L1
Mouse Recombinant Monoclonal VL1 antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - Human papillomavirus type 6a, Recombinant fragment - Human papillomavirus type 6a samples.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- LH6L1_D
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Indirect ELISA - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (ab322925), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-6-l1-protein-antibody-lh6l1d-ab322925--indirect-elisa-img441950.jpg "Indirect ELISA - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (ab322925)")
Indirect ELISA - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (ab322925)
Indirect ELISA analysis of ab322925 at 500-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1:1000 dilution dilution.
Antigen: Human Papilloma Virus type 6 (HPV 6) L1 protein (virus like particle).
Antigen concentration: 5000 ng/ml
![Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (ab322925), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-6-l1-protein-antibody-lh6l1d-ab322925--immunocytochemistry-immunofluorescence-img441928.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (ab322925)")
Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (ab322925)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus 6 L1 protein with ab322925 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing nuclear staining in 293T cells transfected with a human papillomavirus type 6B L1, (shown in green) but no staining in 293T cells transfected with a human papillomavirus type 11 L1 expression vector containing a myc tag. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
![Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (ab322925), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-6-l1-protein-antibody-lh6l1d-ab322925--immunocytochemistry-immunofluorescence-img441927.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (ab322925)")
Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] (ab322925)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus 6 L1 protein with ab322925 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing nuclear staining in 293T cells transfected with a human papillomavirus type 6B L1 expression vector containing a myc tag (shown in green) but no staining in 293T cells transfected with a human papillomavirus type 18 L1 or human papillomavirus type 4 L1 expression vector containing a myc tag. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
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