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AB324811

Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] - BSA and Azide free

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Mouse Recombinant Monoclonal VL1 antibody. Carrier free. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - Human papillomavirus type 6a, Recombinant fragment - Human papillomavirus type 6a samples.

View Alternative Names

Major capsid protein L1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] - BSA and Azide free (AB324811)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] - BSA and Azide free (AB324811)

This data was developed using ab322925, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus 6 L1 protein with ab322925 at 1/2000 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a human papillomavirus type 6B L1 expression vector containing a myc tag (shown in green) but no staining in 293T cells transfected with a human papillomavirus type 18 L1 or human papillomavirus type 4 L1 expression vector containing a myc tag. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] - BSA and Azide free (AB324811)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] - BSA and Azide free (AB324811)

This data was developed using ab322925, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus 6 L1 protein with ab322925 at 1/2000 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a human papillomavirus type 6B L1, (shown in green) but no staining in 293T cells transfected with a human papillomavirus type 11 L1 expression vector containing a myc tag. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Indirect ELISA - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] - BSA and Azide free (AB324811)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] - BSA and Azide free (AB324811)

This data was developed using ab322925, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322925 at 500-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Human Papilloma Virus type 6 (HPV 6) L1 protein (virus like particle).

Antigen concentration : 5000 ng/ml

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LH6L1_D

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human papillomavirus type 6a

Applications

ICC/IF, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human papillomavirus type 6a": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Human papillomavirus type 6a": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell line - Human papillomavirus type 6a": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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Product details

ab324811 is the carrier-free version of ab322925.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
See full target information L1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com