Mouse Monoclonal Minor capsid protein L2 antibody. Suitable for ICC/IF and reacts with Transfected cell line - Human papillomavirus type 18 samples.
IgG1
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC/IF | |
---|---|
Human papillomavirus type 18 | Predicted |
Transfected cell line - Human papillomavirus type 18 | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human papillomavirus type 18 | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human papillomavirus type 18 | Dilution info - | Notes - |
Minor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, L2 escorts the genomic DNA into the nucleus by promoting escape from the endosomal compartments and traffic through the host Golgi network. Mechanistically, the C-terminus of L2 possesses a cell-penetrating peptide that protudes from the host endosome, interacts with host cytoplasmic retromer cargo and thereby mediates the capsid delivery to the host trans-Golgi network. Plays a role through its interaction with host dynein in the intracellular microtubule-dependent transport of viral capsid toward the nucleus. Mediates the viral genome import into the nucleus through binding to host importins. Once within the nucleus, L2 localizes viral genomes to host PML bodies in order to activate early gene expression for establishment of infection. Later on, promotes late gene expression by interacting with the viral E2 protein and by inhibiting its transcriptional activation functions. During virion assembly, encapsidates the genome by direct interaction with the viral DNA.
Minor capsid protein L2, L2
Mouse Monoclonal Minor capsid protein L2 antibody. Suitable for ICC/IF and reacts with Transfected cell line - Human papillomavirus type 18 samples.
IgG1
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
Affinity purification Protein A
The immunogen used to generate this antibody is targeting the HPV18 L2 protein. This clone cross-react with HPV16 L2, HPV31 L2, HPV45 L2, HPV51 L2, HPV6B L2, and HPV11 L2 proteins.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus L2 protein with ab322523 at 1/1000 (1.04 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a Human papillomavirus type 18 L2 protein, a Human papillomavirus type 16 L2 protein, a Human papillomavirus type 31 L2 protein, a Human papillomavirus type 45 L2 protein, a Human papillomavirus type 51 L2 protein, a Human papillomavirus type 6B L2 protein and a Human papillomavirus type 11 L2 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com