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AB317111

Anti-Human Papillomavirus type 16 E7 antibody [HL1821]

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Rabbit Monoclonal VE7 antibody. Suitable for WB, sELISA, LFA and reacts with Transfected cell lysate - Human papillomavirus type 16, Human papillomavirus type 16 samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human papillomavirus type 16 Protein E7.

View Alternative Names

Protein E7, E7

6 Images
Sandwich ELISA - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)

Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein His tag using antibodies : Capture : Human Papillomavirus type 16 E7 antibody (5 ug/mL) and Detection : HRP-conjugated ab317111 (1 ug/mL).

Please notice that ab317111 needs to be conjugated to HRP to function as the detection antibody.

Sandwich ELISA - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)

Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein using antibodies : Capture : ab317111 (5 ug/mL) and Detection : HRP-conjugated Human Papillomavirus type 16 E7 antibody (1 ug/mL).

Western blot - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)
  • WB

Supplier Data

Western blot - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE and the membrane was blotted with ab317111 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (ab317111) at 1/500 dilution

Lane 1:

CaSki whole cell extracts at 30 µg

Lane 2:

SiHa whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)
  • WB

Supplier Data

Western blot - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE and the membrane was blotted with ab317111 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (ab317111) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged HPV type 16 E7 transfected (+) 293T whole cell extracts at 30 µg

Lane 3:

DDDDK-tagged HPV type 18 E7 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)
  • WB

Supplier Data

Western blot - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)

Human papillomavirus protein (100 ng) were separated by 15% SDS-PAGE and the membrane was blotted with ab317111 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (ab317111) at 1/1000 dilution

Lane 1:

Human papillomavirus protein (HPV16 E7) at 100 ng

Lane 2:

Human papillomavirus protein (HPV18 E7) at 100 ng

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Lateral flow assay - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)
  • LFA

Supplier Data

Lateral flow assay - Anti-Human Papillomavirus type 16 E7 antibody [HL1821] (AB317111)

Detection of Human Papillomavirus type 16 E7 protein by lateral flow assay using the monoclonal antibody pair.

Capture : Human Papillomavirus type 16 E7 antibody
Detection : ab317111

Samples (10 ng) :
1. HPV16 E7 protein
2. HPV18 E7 protein
3. Lysis buffer

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1821

Isotype

IgG

Carrier free

No

Reacts with

Human papillomavirus type 16

Applications

sELISA, LFA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human papillomavirus type 16 Protein E7.

P03129

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "LFA" : {"fullname" : "Lateral flow assay", "shortname":"LFA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human papillomavirus type 16": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>", "LFA-species-checked": "testedAndGuaranteed", "LFA-species-dilution-info": "", "LFA-species-notes": "<p></p>" }, "Transfected cell lysate - Human papillomavirus type 16": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "LFA-species-checked": "notRecommended", "LFA-species-dilution-info": "", "LFA-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).
See full target information Protein E7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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