Mouse Monoclonal Minor capsid protein L2 antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - human papillomavirus 31, Recombinant fragment - human papillomavirus 31 samples.
Images
![Indirect ELISA - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (AB322520), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-type-31-l2-antibody-k3hpv31l220-38-ab322520--indirect-elisa-img440389.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (AB322520)")
![Western blot - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (AB322520), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-type-31-l2-antibody-k3hpv31l220-38-ab322520--western-blot-img440373.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (AB322520)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | I-ELISA | |
|---|---|---|
| human papillomavirus 31 | Predicted | Predicted |
| Recombinant fragment - human papillomavirus 31 | Not recommended | Tested |
| Transfected cell lysate - human papillomavirus 31 | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - human papillomavirus 31 | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species human papillomavirus 31 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - human papillomavirus 31 | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - human papillomavirus 31 | Dilution info 62.5 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species human papillomavirus 31 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - human papillomavirus 31 | Dilution info - | Notes - |
Target data
Function
Minor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, L2 escorts the genomic DNA into the nucleus by promoting escape from the endosomal compartments and traffic through the host Golgi network. Mechanistically, the C-terminus of L2 possesses a cell-penetrating peptide that protudes from the host endosome, interacts with host cytoplasmic retromer cargo and thereby mediates the capsid delivery to the host trans-Golgi network. Plays a role through its interaction with host dynein in the intracellular microtubule-dependent transport of viral capsid toward the nucleus. Mediates the viral genome import into the nucleus through binding to host importins. Once within the nucleus, L2 localizes viral genomes to host PML bodies in order to activate early gene expression for establishment of infection. Later on, promotes late gene expression by interacting with the viral E2 protein and by inhibiting its transcriptional activation functions. During virion assembly, encapsidates the genome by direct interaction with the viral DNA.
Alternative names
Minor capsid protein L2
Mouse Monoclonal Minor capsid protein L2 antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - human papillomavirus 31, Recombinant fragment - human papillomavirus 31 samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Indirect ELISA - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (ab322520), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-type-31-l2-antibody-k3hpv31l220-38-ab322520--indirect-elisa-img440389.jpg "Indirect ELISA - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (ab322520)")
Indirect ELISA - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (ab322520)
Indirect ELISA analysis of ab322520 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1:1000 dilution.
Antigen: HPV Minor capsid protein L2.
Antigen concentration: 5000 ng/ml
![Western blot - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (ab322520), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-type-31-l2-antibody-k3hpv31l220-38-ab322520--western-blot-img440373.jpg "Western blot - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (ab322520)")
Western blot - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (ab322520)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (ab322520) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T transfected with a Human papillomavirus type 31 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 3: 293T transfected with a Human papillomavirus type 33 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 4: 293T transfected with a Human papillomavirus type 16 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 75 kDa, 36 kDa
Exposure time: 6s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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