Mouse Recombinant Monoclonal Minor capsid protein L2 antibody. Suitable for ICC/IF, I-ELISA, WB and reacts with Transfected cell line - Human papillomavirus 4, Recombinant fragment - Human papillomavirus 4, Transfected cell lysate - Human papillomavirus 4 samples.
Images
![Indirect ELISA - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-type-4-l2-antibody-hpv4l220-384sa-ab322958--indirect-elisa-img441951.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)")
![Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-type-4-l2-antibody-hpv4l220-384sa-ab322958--immunocytochemistry-immunofluorescence-img441930.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)")
![Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-type-4-l2-antibody-hpv4l220-384sa-ab322958--western-blot-img441924.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (AB322958)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | I-ELISA | WB | |
|---|---|---|---|
| Human papillomavirus 4 | Predicted | Predicted | Predicted |
| Recombinant fragment - Human papillomavirus 4 | Not recommended | Tested | Not recommended |
| Transfected cell line - Human papillomavirus 4 | Tested | Not recommended | Not recommended |
| Transfected cell lysate - Human papillomavirus 4 | Not recommended | Not recommended | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human papillomavirus 4 | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus 4 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human papillomavirus 4, Transfected cell lysate - Human papillomavirus 4 | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human papillomavirus 4 | Dilution info 1000 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus 4 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human papillomavirus 4, Transfected cell lysate - Human papillomavirus 4 | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human papillomavirus 4 | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus 4 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human papillomavirus 4, Recombinant fragment - Human papillomavirus 4 | Dilution info - | Notes - |
Target data
Function
Minor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, L2 escorts the genomic DNA into the nucleus by promoting escape from the endosomal compartments and traffic through the host Golgi network. Mechanistically, the C-terminus of L2 possesses a cell-penetrating peptide that protudes from the host endosome, interacts with host cytoplasmic retromer cargo and thereby mediates the capsid delivery to the host trans-Golgi network. Plays a role through its interaction with host dynein in the intracellular microtubule-dependent transport of viral capsid toward the nucleus. Mediates the viral genome import into the nucleus through binding to host importins. Once within the nucleus, L2 localizes viral genomes to host PML bodies in order to activate early gene expression for establishment of infection. Later on, promotes late gene expression by interacting with the viral E2 protein and by inhibiting its transcriptional activation functions. During virion assembly, encapsidates the genome by direct interaction with the viral DNA.
Additional Targets
L2
Alternative names
Minor capsid protein L2
Mouse Recombinant Monoclonal Minor capsid protein L2 antibody. Suitable for ICC/IF, I-ELISA, WB and reacts with Transfected cell line - Human papillomavirus 4, Recombinant fragment - Human papillomavirus 4, Transfected cell lysate - Human papillomavirus 4 samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- HPV4L2(20-38)_4SA
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody cross-react with overexpressed human papillomavirus type 4 L2 protein and human papillomavirus type 65 L2 protein.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Indirect ELISA - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-type-4-l2-antibody-hpv4l220-384sa-ab322958--indirect-elisa-img441951.jpg "Indirect ELISA - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958)")
Indirect ELISA - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958)
Indirect ELISA analysis of ab322958 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1:1000 dilution.
Antigen: Human papillomavirus 4 Minor capsid protein L2.
Antigen concentration: 5000 ng/ml
![Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-type-4-l2-antibody-hpv4l220-384sa-ab322958--immunocytochemistry-immunofluorescence-img441930.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958)")
Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HPV4 L2 with ab322958 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing nuclear staining in 293T cells transfected with a Human papillomavirus type 4 L2 and Human papillomavirus type 65 L2 expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a human papillomavirus type 60 L2 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
![Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958), expandable thumbnail](https://content.abcam.com/products/images/human-papillomavirus-type-4-l2-antibody-hpv4l220-384sa-ab322958--western-blot-img441924.jpg "Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958)")
Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (ab322958) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T transfected with a Human papillomavirus type 4 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 3: 293T transfected with a Human papillomavirus type 65 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 4: 293T transfected with a Human papillomavirus type 60 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 40 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 100 kDa, 36 kDa
Exposure time: 70s
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