Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] - BSA and Azide free (AB324806)

This data was developed using ab322958, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HPV4 L2 with ab322958 at 1/100 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a Human papillomavirus type 4 L2 and Human papillomavirus type 65 L2 expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a human papillomavirus type 60 L2 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] - BSA and Azide free (AB324806)

This data was developed using ab322958, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322958 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : Human papillomavirus 4 Minor capsid protein L2.

Antigen concentration : 5000 ng/ml

• WB

Supplier Data

Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] - BSA and Azide free (AB324806)

This data was developed using ab322958, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] (<a href='/en-us/products/primary-antibodies/human-papillomavirus-type-4-l2-antibody-hpv4l220-384sa-ab322958'>ab322958</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a Human papillomavirus type 4 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 3:

293T transfected with a Human papillomavirus type 65 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 4:

293T transfected with a Human papillomavirus type 60 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 40 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 70s