JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317228

Anti-Human Parechovirus 1+3 VP0 antibody [HL2443]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Parechovirus A1, Transfected cell lysate - Human parechovirus 3, Transfected cell line - Parechovirus A1, Transfected cell line - Human parechovirus 3 samples.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Human Parechovirus 1+3 VP0 antibody [HL2443] (AB317228)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human Parechovirus 1+3 VP0 antibody [HL2443] (AB317228)

ab317228 detects Human Parechovirus type 1/3 VP0 protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : Human Parechovirus type 1/3 VP0 stained by ab317228 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-Human Parechovirus 1+3 VP0 antibody [HL2443] (AB317228)
  • WB

Supplier Data

Western blot - Anti-Human Parechovirus 1+3 VP0 antibody [HL2443] (AB317228)

Non-infected (–) and infected (+) Vero whole cell extracts (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317228 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Human Parechovirus 1+3 VP0 antibody [HL2443] (ab317228) at 1/1000 dilution

Lane 1:

Non-infected (–) Vero whole cell extracts at 30 µg

Lane 2:

HPeV-A3 infected (+) Vero whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Human Parechovirus 1+3 VP0 antibody [HL2443] (AB317228)
  • WB

Supplier Data

Western blot - Anti-Human Parechovirus 1+3 VP0 antibody [HL2443] (AB317228)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317228 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Human Parechovirus 1+3 VP0 antibody [HL2443] (ab317228) at 1/1000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged HPeV-A1 VP0 transfected (+) 293T whole cell extracts at 30 µg

Lane 3:

DDDDK-tagged HPeV-A3 VP0 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2443

Isotype

IgG

Carrier free

No

Reacts with

Parechovirus A1, Human parechovirus 3

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human parechovirus 3": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Parechovirus A1": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human parechovirus 3": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Transfected cell line - Parechovirus A1": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Human parechovirus 3": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Parechovirus A1": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Additional targets

Human Parechovirus 3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com