AB34756

Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8]

Name: Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756) | Abcam
Brand: Abcam Limited
SKU: AB34756
Rating: 3 (2 reviews)

(2 Reviews)

(18 Publications)

Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756) is a mouse monoclonal antibody detecting Human Polyoma virus JCV capsid protein VP1 in Western Blot, ELISA. Suitable for JCV virus.

- Over 10 publications
- Trusted since 2006

View Alternative Names

Major capsid protein VP1, Major structural protein VP1

1 Images

Unknown

Western blot - Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (AB34756)

Immunoblot of recombinant major capsid protein VP1 (500 ng per lane) of human polyomavirus JCV using monoclonal antibody ab34756 at a concentration of 1 μg/mL.

All lanes:

Western blot - Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756)

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8E8

Isotype

IgG2a

Carrier free

Reacts with

JC polyomavirus

Applications

WB, I-ELISA

applications

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "JC polyomavirus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant full length protein - JC polyomavirus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "1/1000 - 1/10000", "IELISA-species-notes": "<p></p>" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), ELISA in JCV virus samples.

Trusted by the scientific community
Anti-Human Polyoma virus JCV capsid protein VP1 [8E8] (ab34756) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with a N-linked glycoprotein containing terminal alpha(2-6)-linked sialic acids on the cell surface to provide virion attachment to target cell. The serotonergic receptor 5HT2AR also acts as a cellular receptor for JCV on human glial cells. Once attached, the virions enter predominantly by a ligand-inducible clathrin-dependent pathway and traffic to the ER. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA at nuclear domains called promyelocytic leukemia (PML) bodies, and participates in rearranging nucleosomes around the viral DNA.

See full target information VP1_POVJC

style

left-column

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302249 PubMed37658522

2023

MicroMagnify: A Multiplexed Expansion Microscopy Method for Pathogens and Infected Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangyu Cheng,Caroline Stefani,Thomas Skillman,Aleksandra Klimas,Aramchan Lee,Emma F DiBernardo,Karina Mueller Brown,Tatyana Milman,Yuhong Wang,Brendan R Gallagher,Katherine Lagree,Bhanu P Jena,Jose S Pulido,Scott G Filler,Aaron P Mitchell,N Luisa Hiller,Adam Lacy-Hulbert,Yongxin Zhao

Viruses 15: PubMed36680132

2022

Should SVGp12 Be Used for JC Polyomavirus Studies? Comment on Prezioso et al. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains. 2022, , 2070.

Applications

Unspecified application

Species

Unspecified reactive species

Stian Henriksen,Christine Hanssen Rinaldo

Viruses 14: PubMed36146876

2022

COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Prezioso,Sara Passerini,Dolores Limongi,Anna Teresa Palamara,Ugo Moens,Valeria Pietropaolo

Cells 10: PubMed34831441

2021

PI3K/AKT/mTOR Signaling Pathway Is Required for JCPyV Infection in Primary Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Michael P Wilczek,Francesca J Armstrong,Colleen L Mayberry,Benjamin L King,Melissa S Maginnis

BMC cancer 21:1141 PubMed34689739

2021

The differential presence of human polyomaviruses, JCPyV and BKPyV, in prostate cancer and benign prostate hypertrophy tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Chenghuang Shen,Chunliang Tung,Chunnun Chao,Yeongchin Jou,Shupei Huang,Menghsiao Meng,Deching Chang,Peilain Chen

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 128:104435 PubMed32442760

2020

Archetype JC polyomavirus DNA associated with extracellular vesicles circulates in human plasma samples.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano Scribano,Mirko Guerrini,Rosaria Arvia,Daniele Guasti,Patrizia Nardini,Paolo Romagnoli,Simone Giannecchini

Journal of virology 94: PubMed31826993

2020

JC Polyomavirus Infection Reveals Delayed Progression of the Infectious Cycle in Normal Human Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Michael P Wilczek,Jeanne K DuShane,Francesca J Armstrong,Melissa S Maginnis

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 20:1188-1191 PubMed31654479

2019

High-grade urothelial carcinoma in a kidney transplant recipient after JC virus nephropathy: The first evidence of JC virus as a potential oncovirus in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Querido,Isabel Fernandes,André Weigert,Sandra Casimiro,Catarina Albuquerque,Sância Ramos,Teresa Adragão,Ivan Luz,Paulo Paixão,Maria Chasqueira,Madalena Santos,Domingos Machado

Annals of neurology 86:878-884 PubMed31600832

2019

Fatal encephalopathy with wild-type JC virus and ruxolitinib therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Bowen Reoma,Christopher Julius Trindade,Maria Chiara Monaco,Jamie Solis,Marta Garcia Montojo,Phuong Vu,Kory Johnson,Erin Beck,Govind Nair,Omar I Khan,Marta Quezado,Stephen M Hewitt,Daniel S Reich,Richard Childs,Avindra Nath

Frontiers in microbiology 10:783 PubMed31065251

2019

High-Throughput Characterization of Viral and Cellular Protein Expression Patterns During JC Polyomavirus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jeanne K DuShane,Michael P Wilczek,Mason A Crocker,Melissa S Maginnis

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8]

Western blot - Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (AB34756)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Complementary Products

Primary Antibodies

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (18)

Product promise