Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26] (AB316207)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling human respiratory syncytial virus A Nucleoprotein with ab316207 at 1/100 (5.3 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human lung. The section was incubated with ab316207 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26] (AB316207)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Human respiratory syncytial virus A Nucleoprotein with ab316207 at 1/100 (5.3 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse lung. The section was incubated with ab316207 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26] (AB316207)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus A Nucleoprotein with ab316207 at 1/4000 (0.133 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a Respiratory Syncytial Virus A Nucleoprotein expression vector containing a myc-His-tag®.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26] (AB316207)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human Respiratory Syncytial Virus A Nucleoprotein expression vector containing a Myc-His tag® (B) HEK-293T transfected with empty vector containing a Myc-His tag® tissue labeling Human respiratory syncytial virus A Nucleoprotein with ab316207 at 1/2000 (0.265 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a human Respiratory Syncytial Virus A Nucleoprotein expression vector containing a Myc-His tag®. No staining on (B) HEK-293T transfected with empty vector containing a Myc-His tag®. The section was incubated with ab316207 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26] (AB316207)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Cell line : Isotype (Left) / 293T cells (human embryonic kidney epithelial cell) transfected with a Respiratory Syncytial Virus A Nucleoprotein expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with a empty expression vector containing a myc-His-tag® (Right) cells labelling Human respiratory syncytial virus A Nucleoprotein with ab316207 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.

• I-ELISA

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Indirect ELISA - Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26] (AB316207)

Indirect ELISA analysis of ab316207 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human respiratory syncytial virus A Nucleoprotein.

Antigen concentration : 5000 ng/ml

• WB

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Western blot - Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26] (AB316207)

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26] (ab316207) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Human respiratory syncytial virus A Nucleoprotein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 43 kDa,15 kDa

false

Exposure time: 10s

• Dot

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Dot Blot - Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26] (AB316207)

Dot blot analysis of Human respiratory syncytial virus A Nucleoprotein using ab316207 at 1 : 1000 (0.53 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody cross-react with human respiratory syncytial virus B (strain B1) Nucleoprotein.

All lanes:

Dot Blot - Anti-Human respiratory syncytial virus A Nucleoprotein antibody [EPR28656-26] (ab316207) at 1/1000 dilution

Lane 1:

His-tagged human respiratory syncytial virus A (strain A2) Nucleoprotein

Lane 2:

His-tagged human respiratory syncytial virus B (strain B1) Nucleoprotein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s