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AB322235

Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15]

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Mouse Recombinant Monoclonal TLS/FUS antibody. Suitable for ICC/IF, I-ELISA, IHC-P and reacts with Transfected cell line - Respiratory syncytial virus, Recombinant fragment - Respiratory syncytial virus samples.

View Alternative Names

Fusion glycoprotein F0, F, Fusion glycoprotein F0, Protein F

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] (AB322235)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] (AB322235)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T transfected with a Human respiratory syncytial virus Fusion (F) Glycoprotein expression vector containing a His-tag cell pellet; (B) HEK-293T transfected with an empty vector containing a His-tag cell pellet labeling Human respiratory syncytial virus Fusion (F) Glycoprotein with ab322235 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a Human respiratory syncytial virus Fusion (F) Glycoprotein expression vector containing a His-tag cell pellet. No staining on (B) HEK-293T transfected with an empty vector containing a His-tag cell pellet.
The section was incubated with ab322235 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] (AB322235)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] (AB322235)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Human respiratory syncytial virus Fusion (F) Glycoprotein with ab322235 at 1/100 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human lung.
The section was incubated with ab322235 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] (AB322235)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] (AB322235)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus Fusion (F) Glycoprotein with ab322235 at 1/2000 (0.56 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a Human respiratory syncytial virus subtype B1 Fusion (F) Glycoprotein expression vector containing a myc-His-tag® and weak staining in 293T cells transfected with a Human respiratory syncytial virus subtype A2 Fusion (F) Glycoprotein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Indirect ELISA - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] (AB322235)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] (AB322235)

Indirect ELISA analysis of ab322235 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : Human respiratory syncytial virus (RSV) (B, strain 18537) Fusion glycoprotein, Human respiratory syncytial virus (RSV) (A, strain RSS-2) Fusion glycoprotein.

Antigen concentration : 5000 ng/ml

  • Carrier free

    Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4.15

Isotype

IgG1

Carrier free

No

Reacts with

Respiratory syncytial virus

Applications

ICC/IF, I-ELISA, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This clone recognises the Fusion (F) Glycoprotein from Human respiratory syncytial virus subtypes A and B.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Respiratory syncytial virus": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "2000 ng/mL", "IELISA-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Respiratory syncytial virus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Transfected cell line - Respiratory syncytial virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Fusion glycoprotein F0. Inactive precursor that is cleaved at two sites by a furin-like protease to give rise to the mature F1 and F2 fusion glycoproteins.. Fusion glycoprotein F1. Class I viral fusion protein (PubMed : 23618766). Under the current model, the protein has at least 3 conformational states : pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state (PubMed : 23618766). During viral and plasma cell membrane fusion, the coiled coil regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain (PubMed : 19966279, PubMed : 23618766). The formation of this structure appears to drive apposition and subsequent fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm (PubMed : 23593008, PubMed : 23618766). This fusion is pH independent and occurs at the plasma or endosomal membrane (Probable). The trimer of F1-F2 (F protein) also facilitates the attachment to host cell by binding to host heparan sulfate (PubMed : 10864656). F protein is involved in the entry into the host cell through the interaction with host IGF1R (PubMed : 32494007). This interaction activates PRKCZ/PKCzeta that recruits host NCL/nucleolin to the apical cell surface where it can bind fusion glycoprotein F1 (PubMed : 21841784, PubMed : 32494007). Later in infection, F protein expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis (PubMed : 10438814). F protein may trigger p53-dependent apoptosis (PubMed : 18216092).. Fusion glycoprotein F2. Major determinant of the species specificity of RSV infection (PubMed : 12663767). The trimer of F1-F2 (F protein) also facilitates the attachment to host cell by binding to host heparan sulfate (PubMed : 10864656). F protein is involved in the entry into the host cell through the interaction with host IGF1R (PubMed : 32494007). This interaction activates PRKCZ/PKCzeta that recruits host NCL/nucleolin to the apical cell surface where it can bind fusion glycoprotein F1 (PubMed : 32494007). Later in infection, F protein expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis (PubMed : 10438814). F protein seems to trigger p53-dependent apoptosis (PubMed : 18216092).
See full target information F

Additional targets

F
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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