Mouse Recombinant Monoclonal PHOSP antibody. Suitable for ICC/IF, I-ELISA, WB and reacts with Transfected cell line - Human respiratory syncytial virus A (strain A2), Transfected cell line - Human respiratory syncytial virus B1, Recombinant fragment - Human respiratory syncytial virus A (strain A2), Recombinant fragment - Human respiratory syncytial virus B1, Transfected cell lysate - Human respiratory syncytial virus A (strain A2), Transfected cell lysate - Human respiratory syncytial virus B1 samples.
Images
![Indirect ELISA - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (AB322893), expandable thumbnail](https://content.abcam.com/products/images/human-respiratory-syncytial-virus-phosphoprotein-vp32-antibody-35-18-ab322893--indirect-elisa-img441948.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (AB322893)")
![Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (AB322893), expandable thumbnail](https://content.abcam.com/products/images/human-respiratory-syncytial-virus-phosphoprotein-vp32-antibody-35-18-ab322893--immunocytochemistry-immunofluorescence-img441926.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (AB322893)")
![Western blot - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (AB322893), expandable thumbnail](https://content.abcam.com/products/images/human-respiratory-syncytial-virus-phosphoprotein-vp32-antibody-35-18-ab322893--western-blot-img441921.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (AB322893)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | I-ELISA | WB | |
|---|---|---|---|
| Human respiratory syncytial virus A (strain A2) | Predicted | Predicted | Predicted |
| Human respiratory syncytial virus B1 | Predicted | Predicted | Predicted |
| Recombinant fragment - Human respiratory syncytial virus A (strain A2) | Not recommended | Tested | Not recommended |
| Recombinant fragment - Human respiratory syncytial virus B1 | Not recommended | Tested | Not recommended |
| Transfected cell line - Human respiratory syncytial virus A (strain A2) | Tested | Not recommended | Not recommended |
| Transfected cell line - Human respiratory syncytial virus B1 | Tested | Not recommended | Not recommended |
| Transfected cell lysate - Human respiratory syncytial virus A (strain A2) | Not recommended | Not recommended | Tested |
| Transfected cell lysate - Human respiratory syncytial virus B1 | Not recommended | Not recommended | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human respiratory syncytial virus A (strain A2) | Dilution info 1/200 | Notes - |
Species Transfected cell line - Human respiratory syncytial virus B1 | Dilution info 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human respiratory syncytial virus A (strain A2), Human respiratory syncytial virus B1 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human respiratory syncytial virus A (strain A2), Recombinant fragment - Human respiratory syncytial virus B1, Transfected cell lysate - Human respiratory syncytial virus A (strain A2), Transfected cell lysate - Human respiratory syncytial virus B1 | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human respiratory syncytial virus A (strain A2) | Dilution info 250 ng/mL | Notes - |
Species Recombinant fragment - Human respiratory syncytial virus B1 | Dilution info 250 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human respiratory syncytial virus A (strain A2), Human respiratory syncytial virus B1 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human respiratory syncytial virus A (strain A2), Transfected cell line - Human respiratory syncytial virus B1, Transfected cell lysate - Human respiratory syncytial virus A (strain A2), Transfected cell lysate - Human respiratory syncytial virus B1 | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human respiratory syncytial virus A (strain A2) | Dilution info 1/1000 | Notes - |
Species Transfected cell lysate - Human respiratory syncytial virus B1 | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human respiratory syncytial virus A (strain A2), Human respiratory syncytial virus B1 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human respiratory syncytial virus A (strain A2), Transfected cell line - Human respiratory syncytial virus B1, Recombinant fragment - Human respiratory syncytial virus A (strain A2), Recombinant fragment - Human respiratory syncytial virus B1 | Dilution info - | Notes - |
Target data
Function
Plays critical roles in regulating RNA replication and transcription through its interactions with multiple proteins (PubMed:25568210, PubMed:26474524). Tethers the RNA-directed RNA polymerase L to the nucleoprotein-RNA complex (PubMed:26474524). Recruits the M2-1 protein, a processivity factor that is required for efficient transcription of viral RNA (PubMed:26474524). Acts as a chaperone for neo-synthesized nucleoprotein by forming an N-P complex that preserves N in a monomeric and RNA-free state and prevents the association of nascent N with host cell RNAs (PubMed:25568210). Recruits the host phosphatase PP1 to inclusion bodies to regulate viral transcription (PubMed:29489893). Together with the nucleoprotein, sequesters host NF-kappa-B in inclusion bodies (IBs) thereby inhibiting this host defense pathway (By similarity).
Additional Targets
P
Alternative names
Phosphoprotein, Protein P, P
Mouse Recombinant Monoclonal PHOSP antibody. Suitable for ICC/IF, I-ELISA, WB and reacts with Transfected cell line - Human respiratory syncytial virus A (strain A2), Transfected cell line - Human respiratory syncytial virus B1, Recombinant fragment - Human respiratory syncytial virus A (strain A2), Recombinant fragment - Human respiratory syncytial virus B1, Transfected cell lysate - Human respiratory syncytial virus A (strain A2), Transfected cell lysate - Human respiratory syncytial virus B1 samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 3.5-18
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Indirect ELISA - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (ab322893), expandable thumbnail](https://content.abcam.com/products/images/human-respiratory-syncytial-virus-phosphoprotein-vp32-antibody-35-18-ab322893--indirect-elisa-img441948.jpg "Indirect ELISA - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (ab322893)")
Indirect ELISA - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (ab322893)
Indirect ELISA analysis of ab322893 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1:1000 dilution dilution.
Antigen: Human respiratory syncytial virus A (strain A2) RSV Phosphoprotein VP32, Human respiratory syncytial virus B (strain B1) RSV Phosphoprotein VP32.
Antigen concentration: 2000 ng/ml
![Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (ab322893), expandable thumbnail](https://content.abcam.com/products/images/human-respiratory-syncytial-virus-phosphoprotein-vp32-antibody-35-18-ab322893--immunocytochemistry-immunofluorescence-img441926.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (ab322893)")
Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (ab322893)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus phosphoprotein VP32 with ab322893 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing positive staining both in 293T cells transfected with a human respiratory syncytial virus A (strain A2) Phosphoprotein VP32 and a human respiratory syncytial virus B (strain B1) Phosphoprotein VP32 expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
![Western blot - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (ab322893), expandable thumbnail](https://content.abcam.com/products/images/human-respiratory-syncytial-virus-phosphoprotein-vp32-antibody-35-18-ab322893--western-blot-img441921.jpg "Western blot - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (ab322893)")
Western blot - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (ab322893)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (ab322893) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a Human respiratory syncytial virus A (strain A2) Phosphoprotein VP32 expression vector containing a myc-his-tag, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a Human respiratory syncytial virus B (strain B1) Phosphoprotein VP32 expression vector containing a myc-his-tag, whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 32 kDa, 124 kDa
Exposure time: 10s
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