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AB324815

Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] - BSA and Azide free

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Mouse Recombinant Monoclonal PHOSP antibody. Carrier free. Suitable for ICC/IF, I-ELISA, WB and reacts with Transfected cell line - Human respiratory syncytial virus A (strain A2), Transfected cell line - Human respiratory syncytial virus B (strain B1), Recombinant fragment - Human respiratory syncytial virus A (strain A2), Recombinant fragment - Human respiratory syncytial virus B (strain B1), Transfected cell lysate - Human respiratory syncytial virus A (strain A2), Transfected cell lysate - Human respiratory syncytial virus B (strain B1) samples.

View Alternative Names

Phosphoprotein, Protein P, P, Phosphoprotein, Protein P

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] - BSA and Azide free (AB324815)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] - BSA and Azide free (AB324815)

This data was developed using ab322893, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus phosphoprotein VP32 with ab322893 at 1/200 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining both in 293T cells transfected with a human respiratory syncytial virus A (strain A2) Phosphoprotein VP32 and a human respiratory syncytial virus B (strain B1) Phosphoprotein VP32 expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Indirect ELISA - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] - BSA and Azide free (AB324815)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] - BSA and Azide free (AB324815)

This data was developed using ab322893, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322893 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Human respiratory syncytial virus A (strain A2) RSV Phosphoprotein VP32, Human respiratory syncytial virus B (strain B1) RSV Phosphoprotein VP32.

Antigen concentration : 2000 ng/ml

Western blot - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] - BSA and Azide free (AB324815)
  • WB

Supplier Data

Western blot - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] - BSA and Azide free (AB324815)

This data was developed using ab322893, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] (<a href='/en-us/products/primary-antibodies/human-respiratory-syncytial-virus-phosphoprotein-vp32-antibody-35-18-ab322893'>ab322893</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Human respiratory syncytial virus A (strain A2) Phosphoprotein VP32 expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Human respiratory syncytial virus B (strain B1) Phosphoprotein VP32 expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 32 kDa,124 kDa

false

Exposure time: 10s

  • Unconjugated

    Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3.5-18

Isotype

IgG1

Carrier free

Yes

Reacts with

Human respiratory syncytial virus A (strain A2), Human respiratory syncytial virus B (strain B1)

Applications

WB, ICC/IF, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human respiratory syncytial virus A (strain A2)": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Human respiratory syncytial virus B (strain B1)": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human respiratory syncytial virus A (strain A2)": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human respiratory syncytial virus B (strain B1)": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Human respiratory syncytial virus A (strain A2)": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Human respiratory syncytial virus B (strain B1)": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human respiratory syncytial virus A (strain A2)": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human respiratory syncytial virus B (strain B1)": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab324815 is the carrier-free version of ab322893.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays critical roles in regulating RNA replication and transcription through its interactions with multiple proteins (PubMed : 25568210, PubMed : 26474524). Tethers the RNA-directed RNA polymerase L to the nucleoprotein-RNA complex (PubMed : 26474524). Recruits the M2-1 protein, a processivity factor that is required for efficient transcription of viral RNA (PubMed : 26474524). Acts as a chaperone for neo-synthesized nucleoprotein by forming an N-P complex that preserves N in a monomeric and RNA-free state and prevents the association of nascent N with host cell RNAs (PubMed : 25568210). Recruits the host phosphatase PP1 to inclusion bodies to regulate viral transcription (PubMed : 29489893). Together with the nucleoprotein, sequesters host NF-kappa-B in inclusion bodies (IBs) thereby inhibiting this host defense pathway (By similarity).
See full target information P

Additional targets

P
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com