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Rabbit Polyclonal Albumin antibody. Suitable for IHC-P, sELISA and reacts with Human samples. Cited in 26 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ALB.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (AB2406), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (AB2406), expandable thumbnail
  • Sandwich ELISA - Anti-Human Serum Albumin antibody (AB2406), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Native Full Length Protein corresponding to Human ALB. Database link P02768

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PsELISA
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

For sandwich ELISA, use this antibody as Detection at 0.5 μg/ml with Mouse monoclonal [15C7] to Human Serum Albumin (Anti-Human Serum Albumin antibody [15C7] ab10241) as Capture.

Associated Products

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Target data

Function

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

Alternative names

Recommended products

Rabbit Polyclonal Albumin antibody. Suitable for IHC-P, sELISA and reacts with Human samples. Cited in 26 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ALB.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Native Full Length Protein corresponding to Human ALB. Database link P02768
Purification technique
Affinity purification Protein A
Light chain type
unknown
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Product promise

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Full details and terms and conditions can be found here:
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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (ab2406), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (ab2406)

    Formalin-fixed, paraffin-embeded human liver tissue stained for Human Serum Albumin using ab2406 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (ab2406), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (ab2406)

    ab2406 staining human normal liver. Staining is localised to the cytoplasm and secreted.
    Left panel: with primary antibody. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be re

  • Sandwich ELISA - Anti-Human Serum Albumin antibody (ab2406), expandable thumbnail

    Sandwich ELISA - Anti-Human Serum Albumin antibody (ab2406)

    Standard Curve for Human Serum Albumin (Analyte: Human Serum Albumin protein (ab67670)); using Capture Antibody Mouse monoclonal [15C7] to Human Serum Albumin (Anti-Human Serum Albumin antibody [15C7] ab10241) at 1μg/ml and Detector Antibody Rabbit polyclonal to Human Serum Albumin (ab2406) .

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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