Rabbit Polyclonal Albumin antibody. Suitable for IHC-P, sELISA and reacts with Human samples. Cited in 26 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ALB.
pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA
IHC-P | sELISA | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes For sandwich ELISA, use this antibody as Detection at 0.5 μg/ml with Mouse monoclonal [15C7] to Human Serum Albumin (Anti-Human Serum Albumin antibody [15C7] ab10241) as Capture. |
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Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin
Rabbit Polyclonal Albumin antibody. Suitable for IHC-P, sELISA and reacts with Human samples. Cited in 26 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ALB.
pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Formalin-fixed, paraffin-embeded human liver tissue stained for Human Serum Albumin using ab2406 at 1/50 dilution in immunohistochemical analysis.
ab2406 staining human normal liver. Staining is localised to the cytoplasm and secreted.
Left panel: with primary antibody. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be re
Standard Curve for Human Serum Albumin (Analyte: Human Serum Albumin protein (ab67670)); using Capture Antibody Mouse monoclonal [15C7] to Human Serum Albumin (Anti-Human Serum Albumin antibody [15C7] ab10241) at 1μg/ml and Detector Antibody Rabbit polyclonal to Human Serum Albumin (ab2406) .
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