AB2406

Anti-Human Serum Albumin antibody

Name: Anti-Human Serum Albumin antibody. Rabbit polyclonal (ab2406) | Abcam
Brand: Abcam Limited
SKU: AB2406
Price: 450 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(33 Publications)

Rabbit Polyclonal Albumin antibody. Suitable for IHC-P, sELISA and reacts with Human samples. Cited in 33 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ALB.

View Alternative Names

GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (AB2406)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (AB2406)

Formalin-fixed, paraffin-embeded human liver tissue stained for Human Serum Albumin using ab2406 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (AB2406)

ab2406 staining human normal liver. Staining is localised to the cytoplasm and secreted.
Left panel : with primary antibody. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be re

Sandwich ELISA - Anti-Human Serum Albumin antibody (AB2406)

sELISA

Unknown

Sandwich ELISA - Anti-Human Serum Albumin antibody (AB2406)

Standard Curve for Human Serum Albumin (Analyte : Human Serum Albumin protein (ab67670)); using Capture Antibody Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) at 1μg/ml and Detector Antibody Rabbit polyclonal to Human Serum Albumin (ab2406).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

IHC-P, sELISA

applications

Immunogen

Native Full Length Protein corresponding to Human ALB.

P02768

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Detection at 0.5 μg/ml with Mouse monoclonal [15C7] to Human Serum Albumin (<a href='/en-us/products/primary-antibodies/human-serum-albumin-antibody-15c7-ab10241'>ab10241</a>) as Capture.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed : 19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).

See full target information ALB

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:111871 PubMed39995868

2025

Single-cell morphological tracking of cell states to identify small-molecule modulators of liver differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca E Graham,Runshi Zheng,Jesko Wagner,Asier Unciti-Broceta,David C Hay,Stuart J Forbes,Victoria L Gadd,Neil O Carragher

Heliyon 10:e38386 PubMed39398063

2024

Unraveling modulation effects on albumin synthesis and inflammation by Striatin, a bioactive protein fraction isolated from : proteomics and approaches.

Applications

Unspecified application

Species

Unspecified reactive species

Affina Musliha,Doni Dermawan,Puji Rahayu,Raymond R Tjandrawinata

Toxicological sciences : an official journal of the Society of Toxicology 199:227-245 PubMed38335931

2024

Development of a human liver microphysiological coculture system for higher throughput chemical safety assessment.

Applications

Unspecified application

Species

Unspecified reactive species

Blanche C Ip,Samantha J Madnick,Sophia Zheng,Tessa C A van Tongeren,Susan J Hall,Hui Li,Suzanne Martin,Sandrine Spriggs,Paul Carmichael,Wei Chen,David Ames,Lori A Breitweiser,Heather E Pence,Andrew J Bowling,Kamin J Johnson,Richard Cubberley,Jeffrey R Morgan,Kim Boekelheide

Advanced healthcare materials 13:e2303785 PubMed38221504

2024

Pump-Less, Recirculating Organ-on-Chip (rOoC) Platform to Model the Metabolic Crosstalk between Islets and Liver.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandra Aizenshtadt,Chencheng Wang,Shadab Abadpour,Pedro Duarte Menezes,Ingrid Wilhelmsen,Andrea Dalmao-Fernandez,Justyna Stokowiec,Alexey Golovin,Mads Johnsen,Thomas M D Combriat,Hanne Røberg-Larsen,Nikolaj Gadegaard,Hanne Scholz,Mathias Busek,Stefan J K Krauss

Advanced healthcare materials 13:e2302502 PubMed37616035

2023

A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Soo-Yeon Kang,Masaki Kimura,Sunil Shrestha,Phillip Lewis,Sangjoon Lee,Yuqi Cai,Pranav Joshi,Prabha Acharya,Jiafeng Liu,Yong Yang,J Guillermo Sanchez,Sriramya Ayyagari,Eben Alsberg,James M Wells,Takanori Takebe,Moo-Yeal Lee

The Journal of steroid biochemistry and molecular biology 232:106355 PubMed37380087

2023

Mass spectrometry reveals that oxysterols are secreted from non-alcoholic fatty liver disease induced organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Sæterdal Kømurcu,Ingrid Wilhelmsen,James L Thorne,Stefan Krauss,Steven Ray Wilson,Aleksandra Aizenshtadt,Hanne Røberg-Larsen

Cell reports methods 3:100440 PubMed37159662

2023

Quantitative chemometric phenotyping of three-dimensional liver organoids by Raman spectral imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Vernon LaLone,Aleksandra Aizenshtadt,John Goertz,Frøydis Sved Skottvoll,Marco Barbero Mota,Junji You,Xiaoyu Zhao,Henriette Engen Berg,Justyna Stokowiec,Minzhi Yu,Anna Schwendeman,Hanne Scholz,Steven Ray Wilson,Stefan Krauss,Molly M Stevens

Lab on a chip 23:591-608 PubMed36655405

2023

Pump-less, recirculating organ-on-a-chip (rOoC) platform.

Applications

Unspecified application

Species

Unspecified reactive species

Mathias Busek,Aleksandra Aizenshtadt,Timo Koch,Anna Frank,Ludivine Delon,Mikel Amirola Martinez,Alexey Golovin,Clotilde Dumas,Justyna Stokowiec,Stefan Gruenzner,Espen Melum,Stefan Krauss

British journal of cancer 127:2186-2197 PubMed36243890

2022

Identification of a high-risk immunogenic prostate cancer patient subset as candidates for T-cell engager immunotherapy and the introduction of a novel albumin-fused anti-CD3 × anti-PSMA bispecific design.

Applications

Unspecified application

Species

Unspecified reactive species

Eske N Glud,Martin Rasmussen,Yonghui Zhang,Ole A Mandrup,Paul Vinu Salachan,Michael Borre,Karina Dalsgaard Sørensen,Kenneth A Howard

Cell stem cell 29:355-371.e10 PubMed35245467

2022

Human biliary epithelial cells from discarded donor livers rescue bile duct structure and function in a mouse model of biliary disease.

Applications

Unspecified application

Species

Unspecified reactive species

John M Hallett,Sofia Ferreira-Gonzalez,Tak Yung Man,Alastair M Kilpatrick,Hannah Esser,Kayleigh Thirlwell,Mark T Macmillan,Daniel Rodrigo-Torres,Benjamin J Dwyer,Victoria L Gadd,Candice Ashmore-Harris,Wei-Yu Lu,John P Thomson,Maurits A Jansen,Eoghan O'Duibhir,Philip J Starkey Lewis,Lara Campana,Rhona E Aird,Thomas S R Bate,Alasdair R Fraser,John D M Campbell,Gabriel C Oniscu,David C Hay,Anthony Callanan,Stuart J Forbes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human Serum Albumin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (AB2406)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (AB2406)

Sandwich ELISA - Anti-Human Serum Albumin antibody (AB2406)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (33)

Product promise