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AB197787

Anti-Hunchback antibody [F18-1G10.2]

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(3 Publications)

Mouse Monoclonal Hunchback antibody. Suitable for ICC/IF and reacts with Drosophila melanogaster samples. Cited in 3 publications.

View Alternative Names

CG9786, hb, Protein hunchback

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Hunchback antibody [F18-1G10.2] (AB197787)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hunchback antibody [F18-1G10.2] (AB197787)

Immunocytochemistry analysis using Hunchback mouse monoclonal F18-1G10 to detect Hunchback protein in all neuroblast nuclei from Drosophila stage 9 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum. The Hunchback stain used mouse monoclonal F18-1G10 at 10 μg/mL and was detected with Alexa555 conjugated goat anti-mouse (at 10 μg/mL).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

F18-1G10.2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Drosophila melanogaster

Applications

ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purity
undefined SDS-PAGE
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Gap class segmentation protein that controls development of head structures.
See full target information hb
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 9: PubMed32511091

2020

The dynamic transmission of positional information in mutants during embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Yang,Hongcun Zhu,Kakit Kong,Xiaoxuan Wu,Jiayi Chen,Peiyao Li,Jialong Jiang,Jinchao Zhao,Bofei Cui,Feng Liu

Bone research 6:34 PubMed30510839

2018

RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Chen,Xin Zhi,Jun Wang,Jiacan Su

Development (Cambridge, England) 144:4552-4562 PubMed29158446

2017

embryonic type II neuroblasts: origin, temporal patterning, and contribution to the adult central complex.

Applications

Unspecified application

Species

Unspecified reactive species

Kathleen T Walsh,Chris Q Doe
View all publications
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