Anti-Huntingtin antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Huntingtin antibody (AB155942)

Immunofluorescent analysis of 293T cells (transfected with Huntingtin23Q and Huntingtin148Q stop constructs ending in amino acid 552), labeling Huntingtin ab155942 at 1/50 dilution.

• ELISA

Supplier Data

ELISA - Anti-Huntingtin antibody (AB155942)

Sandwich Elisa analysis of 293T cells (overexpressing Huntingtin neo epitope 552), labeling Huntingtin protein (0, 0.5,1,1.5 and 2μg) with ab155942 at 1/20 dilution.

• IHC

Unknown

Immunohistochemistry - Anti-Huntingtin antibody (AB155942)

Immunohistochemistry analysis of Mouse cortex tissue samples, labeling Huntingtin with ab155942 at 1/50 dilution (green). Blue : Tissue counterstained with nuclei stain.

• WB

Supplier Data

Western blot - Anti-Huntingtin antibody (AB155942)

Lanes 1-5 are 293Tcells expressing the full length Htt protein and treated with the various caspases. Lanes 6-9 are 293T overexpressing the different Htt fragments to demonstrate the antibody’s specificity to that neo-epitope. Probed with N-terminal pan-Huntingtin antibody or (bottom panel) neoepitope specific rabbit polyclonal antibody.

All lanes:

Western blot - Anti-Huntingtin antibody (ab155942) at 1/500 dilution

Lane 1:

- at 20 µg

Lanes 2 - 4:

Western blot at 20 µg

Lane 5:

<a href='/en-us/products/primary-antibodies/histone-h3-antibody-nuclear-marker-ab18521'>ab18521</a> recognises histone H3 as well as modified histone H3 at approximately 15 kDa(lane1). This is demonstrated by the blocking using the immunising unmodified histone H3 peptide (lane2), histone H3 mono methyl K4 (lane3), histone H3 di methyl K4 (lane4) and histone H3 tri methyl K4 (lane5). at 20 µg

Lane 6:

Huntingtin23Q(1-513) at 20 µg

Lane 7:

Huntingtin23Q(1-536) at 20 µg

Lane 8:

Huntingtin23Q(1-552) at 20 µg

Lane 9:

Huntingtin23Q(1-586) at 20 µg

Predicted band size: 348 kDa

Observed band size: 62 kDa

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