Rabbit Polyclonal Huntingtin phospho S421 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HTT phospho S421 aa 400-450.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HTT phospho S421 aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link P42858
Reactivity data
Select an application| ELISA | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Pig | Predicted | Predicted | Predicted |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes This antibody gave a positive result in ELISA against the immunizing peptide . |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Huntingtin. May play a role in microtubule-mediated transport or vesicle function. Huntingtin, myristoylated N-terminal fragment. Promotes the formation of autophagic vesicles.
Alternative names
HD, IT15, HTT, Huntingtin, Huntington disease protein, HD protein
Recommended products
Rabbit Polyclonal Huntingtin phospho S421 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HTT phospho S421 aa 400-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HTT phospho S421 aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link P42858
- Purification technique
- Affinity purification Immunogen
- Specificity
Detects a 190kDa band in PC-3 cells, which may correspond to Huntingtin. Stains neuronal cytoplasm in immunohistochemistry (the expected localisation). The antibody has not been conclusively tested for specificity for phospho S421 Huntingtin.
This antibody is specific for phosphorylated human Huntington protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntington homologues from chimpanzee, pig and chicken. Cross reactivity with Huntington protein homologues from mouse and rat may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
- Concentration
- Purification notes
Anti-Huntingtin pS421 is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Huntington protein at the pS421 residue.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-Huntingtin (phospho S421) antibody (ab2174)
The 190kDa band may correspond to Huntingtin. However, it is not clear whether the antibody is specific for the phospho S421 form.
All lanes: Western blot - Anti-Huntingtin (phospho S421) antibody (ab2174) at 1/1000 dilution
All lanes: human PC-3 cell lysate
Predicted band size: 348 kDa
Observed band size: 120 kDa, 130 kDa, 190 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Huntingtin (phospho S421) antibody (ab2174)
ab2174 was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. Positive cytoplasmic staining is
observed in neurons. Tissue was formalin-fixed and
paraffin embedded. Detection was with AEC (pink), nuclear counterstaining with Haemotoxylin (blue).
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