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AB157108

Anti-hUPF2/RENT2 antibody

1

(1 Review)

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(1 Publication)

Rabbit Polyclonal hUPF2/RENT2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UPF2 aa 1-50.

View Alternative Names

KIAA1408, RENT2, UPF2, Regulator of nonsense transcripts 2, Up-frameshift suppressor 2 homolog, hUpf2

3 Images
Immunoprecipitation - Anti-hUPF2/RENT2 antibody (AB157108)
  • IP

Unknown

Immunoprecipitation - Anti-hUPF2/RENT2 antibody (AB157108)

Detection of hUPF2/RENT2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157108 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157108 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-hUPF2/RENT2 antibody (ab157108)

Predicted band size: 148 kDa

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Western blot - Anti-hUPF2/RENT2 antibody (AB157108)
  • WB

Unknown

Western blot - Anti-hUPF2/RENT2 antibody (AB157108)

All lanes:

Western blot - Anti-hUPF2/RENT2 antibody (ab157108) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 148 kDa

true

Exposure time: 30s

Western blot - Anti-hUPF2/RENT2 antibody (AB157108)
  • WB

Unknown

Western blot - Anti-hUPF2/RENT2 antibody (AB157108)

All lanes:

Western blot - Anti-hUPF2/RENT2 antibody (ab157108) at 1 µg/mL

Lane 1:

NIH 3T3 whole cell lysate at 50 µg

Lane 2:

TCMK whole cell lysate at 50 µg

Lane 3:

4T1 whole cell lysate at 50 µg

Predicted band size: 148 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human UPF2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9HAU5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Opossum": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab157108 was affinity purified using an epitope specific to hUPF2/RENT2 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The human UPF2 protein also known as RENT2 is a significant component in the mechanism of nonsense-mediated mRNA decay (NMD). This protein weighs approximately 140 kDa and is an important factor in ensuring quality control within mRNA processes. It is expressed widely in various human tissues playing a central role in cellular regulation processes. UPF2 is recognized for its dual role as a scaffold protein and a bridging factor facilitating interactions between other key components of the NMD pathway demonstrating how essential it is for proper mRNA surveillance.
Biological function summary

UPF2 acts as a central component of the NMD complex a critical surveillance system that degrades mRNAs containing premature stop codons. It recruits and interacts with other essential NMD factors like UPF1 and UPF3 forming a complex that ensures correct mRNA splicing and translation. By binding to these proteins UPF2 stabilizes the assembly of the NMD complex playing an important role in recognizing defective mRNAs and initiating their decay. Its efficient operation keeps potentially harmful or non-functional proteins from accumulating within the cell.

Pathways

UPF2 is deeply involved in the nonsense-mediated mRNA decay pathway. This pathway acts as a cellular defense mechanism against aberrant mRNA transcripts. The UPF2 protein collaborates closely with UPF1 an ATP-dependent helicase that triggers the decay process when it detects faulty mRNA. Additionally UPF2 links with other proteins like SMG-1 kinase which phosphorylates UPF1 leading to the activation of NMD. As such UPF2's function within these pathways is integral to maintaining cellular integrity by regulating gene expression.

Dysfunction in UPF2-related activities contributes significantly to certain genetic disorders and cancers. Mutations or malfunctions in the NMD pathway where UPF2 is a major player have been linked to neurological disorders such as some types of intellectual disabilities. In cancers arising defects in NMD can cause the accumulation of oncogenic proteins promoting tumorigenesis. Additionally UPF2 interactions with SMG-1 and UPF1 proteins are critical in managing these processes highlighting its relevance to disease pathogenesis and potential therapeutic interest.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA.
See full target information UPF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 17: PubMed41010453

2025

Probio-M9 Alters the Gut Microbiota and Mitigates Pulmonary Hypertension in a Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Zhao,Gaopeng Li,Kiyomi Ohmichi,Xiaodong Li,Feiyan Zhao,Kaori Ishikawa,Ryou Ishikawa,Kazufumi Nakamura,Naoya Yokota,Zhihong Sun,Lin Hai Kurahara
View all publications
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