Anti-hUPF2/RENT2 antibody

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The human UPF2 protein also known as RENT2 is a significant component in the mechanism of nonsense-mediated mRNA decay (NMD). This protein weighs approximately 140 kDa and is an important factor in ensuring quality control within mRNA processes. It is expressed widely in various human tissues playing a central role in cellular regulation processes. UPF2 is recognized for its dual role as a scaffold protein and a bridging factor facilitating interactions between other key components of the NMD pathway demonstrating how essential it is for proper mRNA surveillance.

Biological function summary

UPF2 acts as a central component of the NMD complex a critical surveillance system that degrades mRNAs containing premature stop codons. It recruits and interacts with other essential NMD factors like UPF1 and UPF3 forming a complex that ensures correct mRNA splicing and translation. By binding to these proteins UPF2 stabilizes the assembly of the NMD complex playing an important role in recognizing defective mRNAs and initiating their decay. Its efficient operation keeps potentially harmful or non-functional proteins from accumulating within the cell.

Pathways

UPF2 is deeply involved in the nonsense-mediated mRNA decay pathway. This pathway acts as a cellular defense mechanism against aberrant mRNA transcripts. The UPF2 protein collaborates closely with UPF1 an ATP-dependent helicase that triggers the decay process when it detects faulty mRNA. Additionally UPF2 links with other proteins like SMG-1 kinase which phosphorylates UPF1 leading to the activation of NMD. As such UPF2's function within these pathways is integral to maintaining cellular integrity by regulating gene expression.

Associated diseases and disorders

Dysfunction in UPF2-related activities contributes significantly to certain genetic disorders and cancers. Mutations or malfunctions in the NMD pathway where UPF2 is a major player have been linked to neurological disorders such as some types of intellectual disabilities. In cancers arising defects in NMD can cause the accumulation of oncogenic proteins promoting tumorigenesis. Additionally UPF2 interactions with SMG-1 and UPF1 proteins are critical in managing these processes highlighting its relevance to disease pathogenesis and potential therapeutic interest.