Rabbit Polyclonal HuR / ELAVL1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ELAVL1 aa 1 to C-terminus.
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Publications
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- Journal of experimental & clinical cancer research : CR 41:1852022SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanhua Du,Xiang Xu,Siang Lv,Han Liu,Hong Sun,Jun WuPubMed 35624501
- Cancer cell international 21:2712021Circ_0000745 strengthens the expression of CCND1 by functioning as miR-488 sponge and interacting with HuR binding protein to facilitate the development of oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKuangzheng Li,Xiaosheng Fan,Ziyi Yan,Jia Zhan,Fangyun Cao,Yixia JiangPubMed 34020639
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ELAVL1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15717
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Associated Products
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Target data
Function
RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462, PubMed:31358969). Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398, PubMed:17632515, PubMed:18285462, PubMed:23519412, PubMed:8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed:29180010).
Alternative names
HUR, ELAVL1, ELAV-like protein 1, Hu-antigen R, HuR
Recommended products
Rabbit Polyclonal HuR / ELAVL1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ELAVL1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ELAVL1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15717
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HuR also known as ELAVL1 is a protein that binds RNA in cells. It weighs about 36 kDa and is found in the nucleus and cytoplasm. Its name HuR comes from its similarity to human antigen R. Researchers often call it the HuR protein or ELAVL1 protein. This protein helps stabilize certain mRNA molecules by binding to AU-rich elements (AREs) in their 3' untranslated regions. Its binding affects mRNA decay rates and therefore influences protein synthesis.
- Biological function summary
HuR influences cell growth differentiation and stress responses. It does not work alone; it often forms part of larger ribonucleoprotein complexes. By stabilizing mRNAs HuR ensures the continuous production of proteins necessary for cell survival and adaptation to stressors. This regulatory role highlights its involvement in many cellular processes making it a focus for study in relation to cellular homeostasis.
- Pathways
HuR is part of the mRNA surveillance pathway and the response to cellular stress. HuR modulates gene expression by interacting with proteins involved in transcription and translation regulation. It frequently partners with proteins like TTP (tristetraprolin) that also bind to AREs affecting mRNA stability and translation processes. Such interactions extend HuR's influence across pathways related to gene expression regulation and response to stress.
- Associated diseases and disorders
Researchers associate HuR with cancer and neurodegenerative diseases. In cancer HuR stabilizes mRNAs of many oncogenes aiding tumor growth and progression. It may interact with proteins like p53 affecting tumor suppressor pathways. For neurodegenerative diseases HuR's role in mRNA stability can influence neural health connected to proteins like TDP-43 in conditions such as Alzheimer's and ALS. Understanding HuR's function offers insights into potential therapeutic targets for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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14 product images
Western blot - Anti-HuR / ELAVL1 antibody (ab238528)
Blocking buffer: 3% nonfat dry milk in TBST.All lanes: Western blot - Anti-HuR / ELAVL1 antibody (ab238528) at 1/1000 dilution
Lane 1: Wild-type HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 25 µg
Lane 2: HuR / ELAVL1 knockout HEK-293 cell lysate at 25 µg
SecondaryAll lanes: HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 90s
Western blot - Anti-HuR / ELAVL1 antibody (ab238528)
Blocking buffer: 3% non-fat dry milk in TBST.All lanes: Western blot - Anti-HuR / ELAVL1 antibody (ab238528) at 1/1000 dilution
Lane 1: U-251 MG (Human brain glioma cell line) cell extracts at 25 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts at 25 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) cell extracts at 25 µg
Lane 4: K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extracts at 25 µg
Lane 5: PC-3 (Human prostate adenocarcinoma cell line) cell extracts at 25 µg
SecondaryAll lanes: HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded human colon tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded rat testis tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded mouse testis tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded rat brain tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded mouse kidney tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded human esophagus tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded human esophageal cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded human stomach tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded human gastric cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded human kidney tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded human kidney cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)
Paraffin-embedded rat cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.
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