AB238528

Anti-HuR / ELAVL1 antibody

Name: Anti-HuR / ELAVL1 antibody (ab238528) | Abcam
Brand: Abcam Limited
SKU: AB238528

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(5 Publications)

Rabbit Polyclonal HuR / ELAVL1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ELAVL1 aa 1 to C-terminus.

View Alternative Names

HUR, ELAVL1, ELAV-like protein 1, Hu-antigen R, HuR

14 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human gastric cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human esophagus tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human colon tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human kidney tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human kidney cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human esophageal cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human stomach tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded rat cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded rat testis tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded rat brain tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded mouse testis tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded mouse kidney tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-HuR / ELAVL1 antibody (AB238528)

Blocking buffer : 3% non-fat dry milk in TBST.

All lanes:

Western blot - Anti-HuR / ELAVL1 antibody (ab238528) at 1/1000 dilution

Lane 1:

U-251 MG (Human brain glioma cell line) cell extracts at 25 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts at 25 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell extracts at 25 µg

Lane 4:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extracts at 25 µg

Lane 5:

PC-3 (Human prostate adenocarcinoma cell line) cell extracts at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-HuR / ELAVL1 antibody (AB238528)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-HuR / ELAVL1 antibody (ab238528) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 25 µg

Lane 2:

HuR / ELAVL1 knockout HEK-293 cell lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 36 kDa

true

Exposure time: 90s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ELAVL1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15717

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HuR also known as ELAVL1 is a protein that binds RNA in cells. It weighs about 36 kDa and is found in the nucleus and cytoplasm. Its name HuR comes from its similarity to human antigen R. Researchers often call it the HuR protein or ELAVL1 protein. This protein helps stabilize certain mRNA molecules by binding to AU-rich elements (AREs) in their 3' untranslated regions. Its binding affects mRNA decay rates and therefore influences protein synthesis.

Biological function summary

HuR influences cell growth differentiation and stress responses. It does not work alone; it often forms part of larger ribonucleoprotein complexes. By stabilizing mRNAs HuR ensures the continuous production of proteins necessary for cell survival and adaptation to stressors. This regulatory role highlights its involvement in many cellular processes making it a focus for study in relation to cellular homeostasis.

Pathways

HuR is part of the mRNA surveillance pathway and the response to cellular stress. HuR modulates gene expression by interacting with proteins involved in transcription and translation regulation. It frequently partners with proteins like TTP (tristetraprolin) that also bind to AREs affecting mRNA stability and translation processes. Such interactions extend HuR's influence across pathways related to gene expression regulation and response to stress.

Researchers associate HuR with cancer and neurodegenerative diseases. In cancer HuR stabilizes mRNAs of many oncogenes aiding tumor growth and progression. It may interact with proteins like p53 affecting tumor suppressor pathways. For neurodegenerative diseases HuR's role in mRNA stability can influence neural health connected to proteins like TDP-43 in conditions such as Alzheimer's and ALS. Understanding HuR's function offers insights into potential therapeutic targets for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed : 14517288, PubMed : 18285462, PubMed : 31358969). Involved in embryonic stem cell (ESC) differentiation : preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed : 32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398, PubMed : 17632515, PubMed : 18285462, PubMed : 23519412, PubMed : 8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed : 8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed : 29180010).

See full target information ELAVL1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:33763 PubMed41028834

2025

Icariin inhibits proliferation and migration of VSMCs via LncRNA H19 competitively binding to HuR.

Applications

Unspecified application

Species

Unspecified reactive species

Yibing Zhang,Peng Huang,Min Li,Zhimin Fan

International journal of molecular medicine 56: PubMed40937577

2025

CDC5L binds to ELAVL1 to inhibit pyroptosis in hepatocellular carcinoma through the Caspase 3/GSDME signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Liang,Zhongcheng Zhu,Yangshuo Tang,Shuhua Zhou,Moyan Xiao,Xuejun Gong,Ke Ye

Genes 14: PubMed37510387

2023

Functional Characteristics of Diverse Mutations Associated with Isolated Foveal Hypoplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Itsuka Matsushita,Hiroto Izumi,Shinji Ueno,Takaaki Hayashi,Kaoru Fujinami,Kazushige Tsunoda,Takeshi Iwata,Yoshiaki Kiuchi,Hiroyuki Kondo

Journal of experimental & clinical cancer research : CR 41:185 PubMed35624501

2022

SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhua Du,Xiang Xu,Siang Lv,Han Liu,Hong Sun,Jun Wu

Cancer cell international 21:271 PubMed34020639

2021

Circ_0000745 strengthens the expression of CCND1 by functioning as miR-488 sponge and interacting with HuR binding protein to facilitate the development of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kuangzheng Li,Xiaosheng Fan,Ziyi Yan,Jia Zhan,Fangyun Cao,Yixia Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HuR / ELAVL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Western blot - Anti-HuR / ELAVL1 antibody (AB238528)

Western blot - Anti-HuR / ELAVL1 antibody (AB238528)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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