AB28660

Anti-HuR / ELAVL1 antibody

(2 Reviews)

(6 Publications)

Rabbit Polyclonal HuR / ELAVL1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

HUR, ELAVL1, ELAV-like protein 1, Hu-antigen R, HuR

2 Images

Project

Western blot - Anti-HuR / ELAVL1 antibody (AB28660)

All lanes:

Western blot - Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - Jurkat whole cell lysate (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 20 µg

Lane 3:

Western blot - A-431 whole cell lysate (<a href='/en-us/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa,52 kDa

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Lab

Western blot - Anti-HuR / ELAVL1 antibody (AB28660)

False colour image of Western blot : Anti-HuR / ELAVL1 antibody staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab28660 was shown to bind specifically to HuR / ELAVL1. A band was observed at 36 kDa in wild-type SW480 cell lysates with no signal observed at this size in ELAVL1 knockout cell line ab270485 (knockout cell lysate ab270508). To generate this image, wild-type and ELAVL1 knockout SW480 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^Â® 20 (TBS-T) before incubation with primary antibodies overnight at 4 Â°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^Â® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^Â® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/mL

Lane 1:

Wild-type SW480 cell lysate at 20 µg

Lane 2:

ELAVL1 knockout SW480 cell lysate at 20 µg

Lane 2:

Western blot - Human ELAVL1 knockout SW480 cell line (<a href='/en-us/products/cell-lines/human-elavl1-knockout-sw480-cell-line-ab270485'>ab270485</a>)

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

HCT 116 cell lysate at 20 µg

Predicted band size: 36 kDa

Observed band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Another band of approximately 52 kDa is also detected, we are unsure as the identity of this band.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HuR also known as ELAVL1 is a protein that binds RNA in cells. It weighs about 36 kDa and is found in the nucleus and cytoplasm. Its name HuR comes from its similarity to human antigen R. Researchers often call it the HuR protein or ELAVL1 protein. This protein helps stabilize certain mRNA molecules by binding to AU-rich elements (AREs) in their 3' untranslated regions. Its binding affects mRNA decay rates and therefore influences protein synthesis.

Biological function summary

HuR influences cell growth differentiation and stress responses. It does not work alone; it often forms part of larger ribonucleoprotein complexes. By stabilizing mRNAs HuR ensures the continuous production of proteins necessary for cell survival and adaptation to stressors. This regulatory role highlights its involvement in many cellular processes making it a focus for study in relation to cellular homeostasis.

Pathways

HuR is part of the mRNA surveillance pathway and the response to cellular stress. HuR modulates gene expression by interacting with proteins involved in transcription and translation regulation. It frequently partners with proteins like TTP (tristetraprolin) that also bind to AREs affecting mRNA stability and translation processes. Such interactions extend HuR's influence across pathways related to gene expression regulation and response to stress.

Researchers associate HuR with cancer and neurodegenerative diseases. In cancer HuR stabilizes mRNAs of many oncogenes aiding tumor growth and progression. It may interact with proteins like p53 affecting tumor suppressor pathways. For neurodegenerative diseases HuR's role in mRNA stability can influence neural health connected to proteins like TDP-43 in conditions such as Alzheimer's and ALS. Understanding HuR's function offers insights into potential therapeutic targets for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed : 14517288, PubMed : 18285462, PubMed : 31358969). Involved in embryonic stem cell (ESC) differentiation : preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed : 32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398, PubMed : 17632515, PubMed : 18285462, PubMed : 23519412, PubMed : 8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed : 8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed : 29180010).

See full target information ELAVL1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular and cellular cardiology 174:38-46 PubMed36372279

2022

HuR-dependent expression of Wisp1 is necessary for TGFβ-induced cardiac myofibroblast activity.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa C Green,Samuel Slone,Sarah R Anthony,Adrienne R Guarnieri,Sharon Parkins,Shannon M Shearer,Michelle L Nieman,Sudeshna Roy,Jeffrey Aube,Xiaoqing Wu,Liang Xu,Onur Kanisicak,Michael Tranter

Genes & development 36:737-751 PubMed35798383

2022

Primary cilia control translation and the cell cycle in medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Ha Youn,Shirui Hou,Chang-Chih Wu,Daisuke Kawauchi,Brent A Orr,Giles W Robinson,David Finkelstein,Makoto M Taketo,Richard J Gilbertson,Martine F Roussel,Young-Goo Han

Cell death discovery 6:72 PubMed32802409

2020

Circular RNA regulates ferroptosis in HCC cells through interacting with RNA binding protein ALKBH5.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqian Liu,Qi Wang,Xin Wang,Zongzhen Xu,Xiaoqing Wei,Jie Li

JCI insight 4: PubMed30668549

2019

Human antigen R as a therapeutic target in pathological cardiac hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa C Green,Sarah R Anthony,Samuel Slone,Lindsey Lanzillotta,Michelle L Nieman,Xiaoqing Wu,Nathan Robbins,Shannon M Jones,Sudeshna Roy,A Phillip Owens,Jeffrey Aube,Liang Xu,John N Lorenz,Burns C Blaxall,Jack Rubinstein,Joshua B Benoit,Michael Tranter

Cell research 27:416-439 PubMed28244490

2017

The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wang,Wei Song,Hongmei Zhao,Yanni Ma,Yuxia Li,Di Zhai,Jingnan Pi,Yanmin Si,Jiayue Xu,Lei Dong,Rui Su,Mengmeng Zhang,Yong Zhu,Xiaoxia Ren,Fei Miao,Wenjie Liu,Feng Li,Junwu Zhang,Aibin He,Ge Shan,Jingyi Hui,Linfang Wang,Jia Yu

The Journal of biological chemistry 289:3703-12 PubMed24362020

2013

U1A regulates 3' processing of the survival motor neuron mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Eileen Workman,Alex Veith,Daniel J Battle

View all publications

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Product promise

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Anti-HuR / ELAVL1 antibody

Western blot - Anti-HuR / ELAVL1 antibody (AB28660)

Western blot - Anti-HuR / ELAVL1 antibody (AB28660)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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