Anti-HuR / ELAVL1 antibody [EPR17397]
- RabMAb
- Recombinant
- KO Validated
- 20ul selling size
- What is this?
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(94 Publications)
Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) is a rabbit monoclonal antibody detecting HuR / ELAVL1 in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
View Alternative Names
HUR, ELAVL1, ELAV-like protein 1, Hu-antigen R, HuR
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HuR / ELAVL1 with ab200342 at 1/500. Cells were fixed with 100% methanol. ab150077 an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control : PBS only.
Nuclear counter stain : DAPI.
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
ab200342 staining HuR / ELAVL1 in Jurkat (human acute T cell leukemia) cellsby intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/23000. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control : Rabbit monoclonal IgG (Black)
Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)
- IP
Lab
Immunoprecipitation - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
HuR / ELAVL1 was immunoprecipitated from 293T (human embryonic kidney epithelial cell) whole cell lysate with ab200342 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200342 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (ab131366) was used as secondary antibody at 1/5000 dilution.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/30 dilution
Lane 1:
293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg
Lane 2:
293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3:
Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab200342 in 293T whole cell lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Observed band size: 36 kDa
false
Exposure time: 8s
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on rat cerebral cortex tissue tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Lab
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
Lanes 1 - 2 : Merged signal (red and green). Green - ab200342 observed at 36 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab200342 was shown to react with ELAVL1 in wild-type SW480 cells in western blot with loss of signal observed in ELAVL1 knockout sample. Wild-type and ELAVL1 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab200342 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/5000 dilution
Lane 1:
Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 40 µg
Lane 2:
ELAVL1 knockout SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 40 µg
Lane 2:
Western blot - Human ELAVL1 knockout SW480 cell line (<a href='/en-us/products/cell-lines/human-elavl1-knockout-sw480-cell-line-ab270485'>ab270485</a>)
Predicted band size: 36 kDa
Observed band size: 36 kDa
false
- WB
Supplier Data
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
Blocking/dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilution
Lane 1:
Human fetal brain lysate at 10 µg
Lane 2:
Human fetal kidney lysate at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
false
Exposure time: 5s
- WB
Supplier Data
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
Blocking/dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/5000 dilution
Lane 1:
293 (Human embryonic kidney) whole cell lysate at 20 µg
Lane 2:
HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3:
Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Lane 4:
K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa,48 kDa
Observed band size: 36 kDa
false
Exposure time: 5s
- WB
Supplier Data
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
Blocking/dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilution
All lanes:
Human fetal heart lysate at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
false
Exposure time: 1min
- WB
Supplier Data
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)
Blocking/dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilution
Lane 1:
Mouse heart lysate at 10 µg
Lane 2:
Mouse kidney lysate at 10 µg
Lane 3:
Rat brain lysate at 10 µg
Lane 4:
Rat spleen lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
false
Exposure time: 5s
Related conjugates and formulations (9)
-
Anti-HuR / ELAVL1 antibody [EPR17397] - BSA and Azide free
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660 APC
APC Anti-HuR / ELAVL1 antibody [EPR17397]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-HuR / ELAVL1 antibody [EPR17397]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-HuR / ELAVL1 antibody [EPR17397]
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-HuR / ELAVL1 antibody [EPR17397]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-HuR / ELAVL1 antibody [EPR17397]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-HuR / ELAVL1 antibody [EPR17397]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-HuR / ELAVL1 antibody [EPR17397]
-
578 PE
PE Anti-HuR / ELAVL1 antibody [EPR17397]
Reactivity data
Product details
What is this antibody validated in?
Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of HuR / ELAVL1?
Anti-HuR / ELAVL1 [EPR17397] (ab200342) specifically detects a band for HuR / ELAVL1 (UniProt: Q15717) at a molecular weight of 36kDa.
Trusted by the scientific community
Anti-HuR / ELAVL1 [EPR17397] (ab200342) was first used in a scientific publication in 2015 and has been cited over 50 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) has been confirmed by Western blot testing in ELAVL1 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EPR17397] also available for your convenience: ab200342, Alexa Fluor® 647 - ab209609, Carrier free - ab242410, APC - ab310841, PE - ab310913, Alexa Fluor® 488 - ab310967, Alexa Fluor® 594 - ab311680, Alexa Fluor® 568 - ab312955, Alexa Fluor® 555 - ab313164, Alexa Fluor® 750 - ab321346
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HuR influences cell growth differentiation and stress responses. It does not work alone; it often forms part of larger ribonucleoprotein complexes. By stabilizing mRNAs HuR ensures the continuous production of proteins necessary for cell survival and adaptation to stressors. This regulatory role highlights its involvement in many cellular processes making it a focus for study in relation to cellular homeostasis.
Pathways
HuR is part of the mRNA surveillance pathway and the response to cellular stress. HuR modulates gene expression by interacting with proteins involved in transcription and translation regulation. It frequently partners with proteins like TTP (tristetraprolin) that also bind to AREs affecting mRNA stability and translation processes. Such interactions extend HuR's influence across pathways related to gene expression regulation and response to stress.
Product protocols
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Target data
Publications (94)
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General physiology and biophysics 44:349-361 PubMed40923655
2025
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Neuroreport 36:719-727 PubMed40736326
2025
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Journal of cell communication and signaling 19:e70016 PubMed40401147
2025
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70433 PubMed40085190
2025
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Respiratory research 26:61 PubMed39985019
2025
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The Kaohsiung journal of medical sciences 41:e12949 PubMed39969065
2025
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CytoJournal 21:42 PubMed39737126
2024
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Journal of biochemical and molecular toxicology 38:e70066 PubMed39588760
2024
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NPJ precision oncology 8:243 PubMed39455812
2024
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Journal of biochemical and molecular toxicology 38:e70025 PubMed39445487
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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