Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) is a rabbit monoclonal antibody detecting HuR / ELAVL1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 50 publications

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Related conjugates and formulations

ab242410
Carrier free
Anti-HuR / ELAVL1 antibody [EPR17397] - BSA and Azide free
ab313164
Conjugated
Alexa Fluor® 555 Anti-HuR / ELAVL1 antibody [EPR17397]
ab311680
Conjugated
Alexa Fluor® 594 Anti-HuR / ELAVL1 antibody [EPR17397]
ab310841
Conjugated
APC Anti-HuR / ELAVL1 antibody [EPR17397]
ab310913
Conjugated
PE Anti-HuR / ELAVL1 antibody [EPR17397]
ab312955
Conjugated
Alexa Fluor® 568 Anti-HuR / ELAVL1 antibody [EPR17397]
ab209609
Conjugated
Alexa Fluor® 647 Anti-HuR / ELAVL1 antibody [EPR17397]
ab310967
Conjugated
Alexa Fluor® 488 Anti-HuR / ELAVL1 antibody [EPR17397]
ab321346
Conjugated
Alexa Fluor® 750 Anti-HuR / ELAVL1 antibody [EPR17397]

Images

Flow Cytometry (Intracellular) - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Tested
Rat	Expected	Tested	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 0.1 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information ELAVL1

Function

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462, PubMed:31358969). Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398, PubMed:17632515, PubMed:18285462, PubMed:23519412, PubMed:8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed:29180010).

Alternative names

HUR, ELAVL1, ELAV-like protein 1, Hu-antigen R, HuR

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17397
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of HuR / ELAVL1?

Anti-HuR / ELAVL1 [EPR17397] (ab200342) specifically detects a band for HuR / ELAVL1 (UniProt: Q15717) at a molecular weight of 36kDa.

Trusted by the scientific community

Anti-HuR / ELAVL1 [EPR17397] (ab200342) was first used in a scientific publication in 2015 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) has been confirmed by Western blot testing in ELAVL1 Knockout HAP1 cells.

Other related products

We have a range of other formats of antibody clone [EPR17397] also available for your convenience:
ab200342, Alexa Fluor® 647 - ab209609, Carrier free - ab242410, APC - ab310841, PE - ab310913, Alexa Fluor® 488 - ab310967, Alexa Fluor® 594 - ab311680, Alexa Fluor® 568 - ab312955, Alexa Fluor® 555 - ab313164, Alexa Fluor® 750 - ab321346

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HuR also known as ELAVL1 is a protein that binds RNA in cells. It weighs about 36 kDa and is found in the nucleus and cytoplasm. Its name HuR comes from its similarity to human antigen R. Researchers often call it the HuR protein or ELAVL1 protein. This protein helps stabilize certain mRNA molecules by binding to AU-rich elements (AREs) in their 3' untranslated regions. Its binding affects mRNA decay rates and therefore influences protein synthesis.

Biological function summary

HuR influences cell growth differentiation and stress responses. It does not work alone; it often forms part of larger ribonucleoprotein complexes. By stabilizing mRNAs HuR ensures the continuous production of proteins necessary for cell survival and adaptation to stressors. This regulatory role highlights its involvement in many cellular processes making it a focus for study in relation to cellular homeostasis.

Pathways

HuR is part of the mRNA surveillance pathway and the response to cellular stress. HuR modulates gene expression by interacting with proteins involved in transcription and translation regulation. It frequently partners with proteins like TTP (tristetraprolin) that also bind to AREs affecting mRNA stability and translation processes. Such interactions extend HuR's influence across pathways related to gene expression regulation and response to stress.

Associated diseases and disorders

Researchers associate HuR with cancer and neurodegenerative diseases. In cancer HuR stabilizes mRNAs of many oncogenes aiding tumor growth and progression. It may interact with proteins like p53 affecting tumor suppressor pathways. For neurodegenerative diseases HuR's role in mRNA stability can influence neural health connected to proteins like TDP-43 in conditions such as Alzheimer's and ALS. Understanding HuR's function offers insights into potential therapeutic targets for these diseases.

Product promise

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11 product images

Flow Cytometry (Intracellular) - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
ab200342 staining HuR / ELAVL1 in Jurkat (human acute T cell leukemia) cellsby intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/23000. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/5000 dilution
Lane 1: 293 (Human embryonic kidney) whole cell lysate at 20 µg
Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Lane 4: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa, 48 kDa
Observed band size: 36 kDa
Exposure time: 5s
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human fetal kidney lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 5s
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilution
All lanes: Human fetal heart lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 1min
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilution
Lane 1: Mouse heart lysate at 10 µg
Lane 2: Mouse kidney lysate at 10 µg
Lane 3: Rat brain lysate at 10 µg
Lane 4: Rat spleen lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 5s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
Lanes 1 - 2: Merged signal (red and green). Green - ab200342 observed at 36 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab200342 was shown to react with ELAVL1 in wild-type SW480 cells in western blot with loss of signal observed in ELAVL1 knockout sample. Wild-type and ELAVL1 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab200342 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/5000 dilution
Lane 1: Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 40 µg
Lane 2: ELAVL1 knockout SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 40 µg
Lane 2: Western blot - Human ELAVL1 knockout SW480 cell line (Human ELAVL1 knockout SW480 cell line ab270485)
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Immunoprecipitation - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
HuR / ELAVL1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200342 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200342 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input). Lane 2: ab200342 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab200342 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
Predicted band size: 36 kDa
Observed band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on rat cerebral cortex tissue tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HuR / ELAVL1 with ab200342 at 1/500. Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.