AB186850

Anti-HUS1 antibody

Name: Anti-HUS1 antibody (ab186850) | Abcam
Brand: Abcam Limited
SKU: AB186850
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal HUS1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HUS1.

View Alternative Names

Checkpoint protein HUS1, hHUS1, HUS1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (AB186850)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (AB186850)

Immunohistochemistry of paraffin-embedded human stomach cancer using ab186850 at dilution of 1 : 200 (x400 lens).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (AB186850)

Immunohistochemistry of paraffin-embedded human kidney using ab186850 at dilution of 1 : 200 (x400 lens).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (AB186850)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186850. Blue DAPI for nuclear staining.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (AB186850)

Immunofluorescence analysis of U2OS cell using ab186850. Blue : DAPI for nuclear staining. DNA damage by a UV-A laser.

Supplier Data

Western blot - Anti-HUS1 antibody (AB186850)

All lanes:

Western blot - Anti-HUS1 antibody (ab186850) at 1/500 dilution

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OVCAR3 cell extract

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K562 cell extract

Lane 3:

293T cell extract

Predicted band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HUS1. The exact immunogen used to generate this antibody is proprietary information.

O60921

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HUS1 also known as checkpoint protein HUS1 homolog is a protein that plays an important role in DNA damage response. It has a molecular mass of about 34 kDa and is expressed in various tissues with notable expression in testis and thymus. HUS1 is part of the Rad9-Rad1-Hus1 (9-1-1) complex which acts as a sensor for DNA damage and activates downstream signaling pathways for DNA repair mechanisms.

Biological function summary

HUS1 is important in maintaining genomic stability and integrity. It forms a complex with RAD9 and RAD1 creating the 9-1-1 complex that localizes to sites of DNA damage. This complex enables the recruitment and activation of several key DNA repair proteins facilitating the repair process. HUS1 plays a role in the activation of cell cycle checkpoints essential for preventing the propagation of damaged DNA during cell division.

Pathways

HUS1 is significantly involved in the DNA damage response and cell cycle regulation pathways. The protein connects into the ATR signaling pathway where it helps activate ATR kinase in response to DNA damage. Related proteins in these pathways include ATM and CHK1 which also serve in coordinating the cellular response to DNA injuries.

HUS1 is associated with cancer pathogenesis particularly in regard to its role in DNA repair. Mutations or dysregulation in HUS1 can lead to genomic instability a hallmark of cancerous cells. In addition to cancer HUS1 plays a role in hereditary diseases such as Ataxia-telangiectasia due to its connection with the ATM protein which is mutated in this disorder. Understanding HUS1's function and interactions might provide insight into therapeutic approaches for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed : 21659603). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed : 21659603). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed : 21659603). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed : 21659603). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed : 21659603).

See full target information HUS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 56:2739-2746 PubMed39643607

2024

GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ha Yeon Jeong,Jin-Sil Park,Jeong Won Choi,Kun Hee Lee,Seung Cheon Yang,Hye Yeon Kang,Sang Hee Cho,Seon-Yeong Lee,A Ram Lee,Youngjae Park,Sung-Hwan Park,Mi-La Cho

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HUS1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (AB186850)

Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (AB186850)

Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (AB186850)

Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (AB186850)

Western blot - Anti-HUS1 antibody (AB186850)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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