AB70161

Anti-HUWE1/Mule antibody

Name: Anti-HUWE1/Mule antibody (ab70161) | Abcam
Brand: Abcam Limited
SKU: AB70161
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(24 Publications)

Rabbit Polyclonal HUWE1/Mule antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 24 publications. Immunogen corresponding to Synthetic Peptide within Human HUWE1 aa 2250 to C-terminus.

View Alternative Names

KIAA0312, KIAA1578, UREB1, HSPC272, HUWE1, E3 ubiquitin-protein ligase HUWE1, ARF-binding protein 1, HECT-type E3 ubiquitin transferase HUWE1, Homologous to E6AP carboxyl terminus homologous protein 9, Large structure of UREB1, Mcl-1 ubiquitin ligase E3, Upstream regulatory element-binding protein 1, ARF-BP1, HectH9, LASU1, Mule, URE-B1, URE-binding protein 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUWE1/Mule antibody (AB70161)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUWE1/Mule antibody (AB70161)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of human ovarian carcinoma tissue labelling HUWE1/Mule with ab70161 at 1/5000 (0.2μg/ml). Detection : DAB.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUWE1/Mule antibody (AB70161)

IHC image of ab70161 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70161, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Supplier Data

Immunoprecipitation - Anti-HUWE1/Mule antibody (AB70161)

HUWE1/Mule was immunoprecipitated from HeLa whole cell lysate with 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab70161 at 1 μg/ml dilution.

Lane 1 : ab70161 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

All lanes:

Immunoprecipitation - Anti-HUWE1/Mule antibody (ab70161)

Predicted band size: 481 kDa

false

Supplier Data

Western blot - Anti-HUWE1/Mule antibody (AB70161)

Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Western blot - Anti-HUWE1/Mule antibody (ab70161) at 1/10000 dilution

Lane 1:

HeLa Whole cell lysate at 50 µg

Lane 2:

HEK293T Whole cell lysate at 50 µg

Lane 3:

Jurkat Whole cell lysate at 50 µg

Predicted band size: 481 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human HUWE1 aa 2250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein HUWE1 also known as Mule Mule HRP or Mule Mouse is a large HECT domain E3 ubiquitin ligase with a molecular weight of approximately 437 kDa. It functions by attaching ubiquitin to substrate proteins marking them for degradation via the proteasome pathway. HUWE1 is expressed ubiquitously in tissues but shows higher expression levels in brain testis and certain cancers. It regulates protein homeostasis highlighting its importance in cellular processes.

Biological function summary

HUWE1/Mule modulates various cellular functions through its ubiquitin ligase activity. It is not a part of a larger complex but interacts with numerous proteins influencing cell cycle regulation apoptosis and DNA repair. Its role in apoptosis involves facilitating the degradation of the pro-apoptotic protein Mcl-1 thereby promoting cell death. Additionally HUWE1 contributes to DNA repair by targeting histone H2AX for modification which is an important step in the DNA damage response.

Pathways

HUWE1 acts within the ubiquitin-proteasome system and the DNA damage response pathway. In the ubiquitin-proteasome system HUWE1 closely interacts with proteins such as p53 and c-Myc playing a significant role in regulating their stability and function. In the DNA damage response pathway the protein's activity affects the signaling required for cell cycle checkpoints underlining its relevance in maintaining genomic stability.

HUWE1 mutations and dysregulation link to intellectual disability and certain cancers. Its association with intellectual disability arises from alterations in neural gene regulation partly through its interaction with proteins like p53. In cancer aberrant activity of HUWE1 results in uncontrolled proliferation as it affects the degradation of critical proteins involved in tumor progression such as c-Myc. Understanding HUWE1's mechanisms can provide insights into these conditions and potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 15567145, PubMed : 15767685, PubMed : 15989957, PubMed : 17567951, PubMed : 18488021, PubMed : 19037095, PubMed : 19713937, PubMed : 20534529, PubMed : 30217973). Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1 (PubMed : 15989957). Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair (PubMed : 19713937). Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4 (PubMed : 15567145, PubMed : 15767685, PubMed : 15989956). Ubiquitinates MFN2 to negatively regulate mitochondrial fusion in response to decreased stearoylation of TFRC (PubMed : 26214738). Ubiquitination of MFN2 also takes place following induction of mitophagy; AMBRA1 acts as a cofactor for HUWE1-mediated ubiquitination (PubMed : 30217973). Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN (PubMed : 18488021). May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation (PubMed : 17567951). Mediates polyubiquitination of isoform 2 of PA2G4 (PubMed : 19037095). Acts in concert with MYCBP2 to regulate the circadian clock gene expression by promoting the lithium-induced ubiquination and degradation of NR1D1 (PubMed : 20534529). Binds to an upstream initiator-like sequence in the preprodynorphin gene (By similarity). Mediates HAPSTR1 degradation, but is also a required cofactor in the pathway by which HAPSTR1 governs stress signaling (PubMed : 35776542). Ubiquitinates PPARA in hepatocytes (By similarity).

See full target information HUWE1

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:271 PubMed40610632

2025

HUWE1 is involved in Sertoli cell polarity establishment by ubiquitination mediated degradation of WT1.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Liu,Mengyue Wang,Changhuo Cen,Zhiming Shen,Jiayi Li,Xiuhong Cui,Xudong Zhao,Min Chen,Fei Gao

Nature communications 15:10594 PubMed39632829

2024

CDK9 recruits HUWE1 to degrade RARα and offers therapeutic opportunities for cutaneous T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Hui Luo,Li-Hong Hu,Jie-Yang Liu,Li Xia,Li Zhou,Ren-Hong Sun,Chen-Cen Lin,Xing Qiu,Biao Jiang,Meng-Ying Yang,Xue-Hong Zhang,Xiao-Bao Yang,Guo-Qiang Chen,Ying Lu

Frontiers in immunology 15:1388517 PubMed39034993

2024

Suppression of interferon α and γ response by Huwe1-mediated Miz1 degradation promotes SARS-CoV-2 replication.

Applications

Unspecified application

Species

Unspecified reactive species

Vinothini Arunagiri,Laura Cooper,Huali Dong,Jake Class,Indrani Biswas,Sujan Vahora,Riddhi Deshpande,Khushi H Gopani,Guochang Hu,Justin M Richner,Lijun Rong,Jing Liu

Molecular systems biology 20:719-740 PubMed38580884

2024

Genome-wide CRISPR screens identify novel regulators of wild-type and mutant p53 stability.

Applications

Unspecified application

Species

Unspecified reactive species

YiQing Lü,Tiffany Cho,Saptaparna Mukherjee,Carmen Florencia Suarez,Nicolas S Gonzalez-Foutel,Ahmad Malik,Sebastien Martinez,Dzana Dervovic,Robin Hyunseo Oh,Ellen Langille,Khalid N Al-Zahrani,Lisa Hoeg,Zhen Yuan Lin,Ricky Tsai,Geraldine Mbamalu,Varda Rotter,Patricia Ashton-Prolla,Jason Moffat,Lucia Beatriz Chemes,Anne-Claude Gingras,Moshe Oren,Daniel Durocher,Daniel Schramek

Nature communications 15:1446 PubMed38365788

2024

PAF1c links S-phase progression to immune evasion and MYC function in pancreatic carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Abdallah Gaballa,Anneli Gebhardt-Wolf,Bastian Krenz,Greta Mattavelli,Mara John,Giacomo Cossa,Silvia Andreani,Christina Schülein-Völk,Francisco Montesinos,Raphael Vidal,Carolin Kastner,Carsten P Ade,Burkhard Kneitz,Georg Gasteiger,Peter Gallant,Mathias Rosenfeldt,Angela Riedel,Martin Eilers

Journal of virology 97:e0128723 PubMed37800948

2023

Oxidative stress sensor Keap1 recognizes HBx protein to activate the Nrf2/ARE signaling pathway, thereby inhibiting hepatitis B virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Adi Ariffianto,Lin Deng,Takayuki Abe,Chieko Matsui,Masahiko Ito,Akihide Ryo,Hussein Hassan Aly,Koichi Watashi,Tetsuro Suzuki,Masashi Mizokami,Yoshiharu Matsuura,Ikuo Shoji

Nature communications 14:152 PubMed36631436

2023

The HAPSTR2 retrogene buffers stress signaling and resilience in mammals.

Applications

Unspecified application

Species

Unspecified reactive species

David R Amici,Harun Cingoz,Milad J Alasady,Sammy Alhayek,Claire M Phoumyvong,Nidhi Sahni,S Stephen Yi,Marc L Mendillo

Frontiers in oncology 12:988528 PubMed36353537

2022

Parthenolide and arsenic trioxide co-trigger autophagy-accompanied apoptosis in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Yi,Xia Gong,Xiao-Yang Yin,Li Wang,Jin-Xia Hou,Jing Chen,Bei Xie,Gang Chen,Li-Na Wang,Xiao-Yuan Wang,Da-Chun Wang,Hu-Lai Wei

Cancer communications (London, England) 42:868-886 PubMed35848447

2022

The E3 ubiquitin ligase HUWE1 acts through the N-Myc-DLL1-NOTCH1 signaling axis to suppress glioblastoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Yuan,Li-Hong Wang,Xian-Xian Zhao,Jiao Wang,Meng-Si Zhang,Qing-Hua Ma,Sen Wei,Ze-Xuan Yan,Yue Cheng,Xiao-Qing Chen,Hong-Bo Zou,Jia Ge,Yan Wang,Xia Zhang,You-Hong Cui,Tao Luo,Xiu-Wu Bian

The Journal of biological chemistry 298:101981 PubMed35472332

2022

E3 ligase HUWE1 promotes PDGF D-mediated osteoblastic differentiation of mesenchymal stem cells by effecting polyubiquitination of β-PDGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Tri Pham,Abdo J Najy,Hyeong-Reh C Kim

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Product promise

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Anti-HUWE1/Mule antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUWE1/Mule antibody (AB70161)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUWE1/Mule antibody (AB70161)

Immunoprecipitation - Anti-HUWE1/Mule antibody (AB70161)

Western blot - Anti-HUWE1/Mule antibody (AB70161)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (24)

Product promise