AB180143

Anti-HYAL1 antibody [EPR5542]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal HYAL1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LUCA1, HYAL1, Hyaluronidase-1, Hyal-1, Hyaluronoglucosaminidase-1, Lung carcinoma protein 1, LuCa-1

1 Images

Supplier Data

Western blot - Anti-HYAL1 antibody [EPR5542] (AB180143)

Blocking buffer and concentration : 5%NFDM/TBST

All lanes:

Western blot - Anti-HYAL1 antibody [EPR5542] (ab180143) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

LnCap cell lysate at 10 µg

Lane 3:

HT-1376 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 60 kDa

false

Carrier free

Anti-HYAL1 antibody [EPR5542] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5542

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HYAL1 also known as hyaluronidase-1 functions as an enzyme that catalyzes the degradation of hyaluronic acid into smaller oligosaccharides. It possesses a mass of approximately 57 kDa. This enzyme is expressed in various tissues including the liver spleen and kidney. HYAL1 plays an essential role in the turnover of extracellular matrix components impacting tissue remodeling and cell migration.

Biological function summary

Hyaluronidase-1 facilitates the breakdown of hyaluronic acid a major component of the extracellular matrix affecting cellular interactions and signaling pathways. HYAL1 does not operate as part of a protein complex but acts individually. Its enzymatic activity influences processes such as cell proliferation tissue repair and inflammatory response as hyaluronic acid fragments modulate these cellular behaviors.

Pathways

Hyaluronidase-1 engages in significant pathways involved in extracellular matrix organization and degradation. It interacts within the hyaluronan metabolism pathway impacting proteins like CD44 and RHAMM which are involved in cell adhesion and motility. By processing hyaluronic acid HYAL1 affects these pathways influencing cellular dynamics and homeostasis.

Abnormal HYAL1 function associates with conditions such as cancer and osteoarthritis. In cancer increased hyaluronidase-1 activity correlates with tumor progression and metastasis as it promotes cancer cell invasion through the extracellular matrix. In osteoarthritis enzymatic degradation of hyaluronic acid disrupts cartilage integrity exacerbating disease symptoms. Additionally the interaction of hyaluronidase-1 with proteins like CD44 in these conditions may further drive pathological changes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.

See full target information HYAL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 26:503 PubMed37822583

2023

Downregulation of lncRNA SATB2‑AS1 facilitates glioma cell proliferation by sponging miR‑671‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Gu,Yongqing Ye,Rauniyar Sunil,Wenjian Zhan,Rutong Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HYAL1 antibody [EPR5542]

Western blot - Anti-HYAL1 antibody [EPR5542] (AB180143)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise