Anti-HYAL2 antibody [EPR28917-156]

8 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYAL2 antibody [EPR28917-156] (ab323609)

  Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling HYAL2 with ab323609 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of human cervical carcinoma. The section was incubated with ab323609 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYAL2 antibody [EPR28917-156] (ab323609)

  Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling HYAL2 with ab323609 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of human lung carcinoma. The section was incubated with ab323609 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYAL2 antibody [EPR28917-156] (ab323609)

  Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling HYAL2 with ab323609 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of human placenta. The section was incubated with ab323609 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYAL2 antibody [EPR28917-156] (ab323609)

  Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling HYAL2 with ab323609 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of human spleen. The section was incubated with ab323609 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Western blot - Anti-HYAL2 antibody [EPR28917-156] (ab323609)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The samples are unboiled.

  All lanes: Western blot - Anti-HYAL2 antibody [EPR28917-156] (ab323609) at 1/1000 dilution

  Lane 1: Human lung cancer tissue lysate at 40 µg

  Lane 2: Human testis tissue lysate at 40 µg

  Lane 3: Human lung tissue lysate at 40 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution

  Observed band size: 54 kDa

  Exposure time: 15s

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  Western blot - Anti-HYAL2 antibody [EPR28917-156] (ab323609)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The samples are unboiled.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-HYAL2 antibody [EPR28917-156] (ab323609) at 1/1000 dilution

  Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 3: JEG-3 (human placenta epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 54 kDa, 36 kDa

  Exposure time: 136s

• 

  Western blot - Anti-HYAL2 antibody [EPR28917-156] (ab323609)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The samples are unboiled.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-HYAL2 antibody [EPR28917-156] (ab323609) at 1/1000 dilution

  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

  Lane 2: HeLa transfected with siRNA specifically targeting HYAL2 whole cell lysate at 20 µg

  Lane 3: Saos-2 (human osteosarcoma epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 54 kDa, 36 kDa

  Exposure time: 136s

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  Immunocytochemistry/ Immunofluorescence - Anti-HYAL2 antibody [EPR28917-156] (ab323609)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% Tween-20 permeabilized Saos-2 (human osteosarcoma epithelial cell) cells labeling HYAL2 with ab323609 at 1/50 (9.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing partial membranous staining in Saos-2 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.