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AB151767

Anti-HYAL3 antibody

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(1 Publication)

Rabbit Polyclonal HYAL3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HYAL3.

View Alternative Names

LUCA3, HYAL3, Hyaluronidase-3, Hyal-3, Hyaluronoglucosaminidase-3, Lung carcinoma protein 3, LuCa-3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYAL3 antibody (AB151767)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYAL3 antibody (AB151767)

Immunohistochemical analysis of paraffin embedded Human mammary cancer tissue, labeling HYAL3 with ab151767 at 1 μg/ml .

Western blot - Anti-HYAL3 antibody (AB151767)
  • WB

Unknown

Western blot - Anti-HYAL3 antibody (AB151767)

All lanes:

Western blot - Anti-HYAL3 antibody (ab151767) at 0.5 µg/mL

Lane 1:

22RV cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

V20S cell lysate

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human HYAL3. The exact immunogen used to generate this antibody is proprietary information.

O43820

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HYAL3 also known as hyaluronoglucosaminidase 3 functions mechanically as an enzyme that catalyzes the degradation of hyaluronan. This protein helps break down hyaluronic acid by cleaving its glycosidic bonds. HYAL3 is expressed in human tissues like lungs liver placenta and skeletal muscle. Its molecular mass is approximately 55 kDa. Researchers recognize HYAL3 as part of a family of hyaluronidase enzymes that play a significant role in maintaining the extracellular matrix.
Biological function summary

Hyaluronoglucosaminidase 3 influences the turnover of hyaluronan in the extracellular environment. Although scientists have not fully clarified its specific biological role due to its relatively weak catalytic activity compared to other hyaluronidases HYAL3 contributes to cellular processes like proliferation differentiation and migration. It participates in a complex with other hyaluronidases such as HYAL1 and HYAL2 which also process hyaluronan and hold a more direct enzymatic role in hyaluronan metabolism.

Pathways

The enzyme hyaluronoglucosaminidase 3 engages in the extracellular matrix (ECM) remodeling pathway. This pathway influences tissue architecture during development and repair. HYAL3 along with hyaluronidases like HYAL1 and HYAL2 regulates hyaluronic acid balance as it affects ECM stability and cell movement. It indirectly supports processes within the Wnt signaling pathway due to its modulatory effects on the ECM further linking it with cellular proliferation and migration.

Studies show that HYAL3 may connect to cancer progression and osteoarthritis. In cancer altered expression of HYAL3 and its related hyaluronidase proteins like HYAL2 may facilitate tumor growth and invasion through ECM remodeling. In osteoarthritis HYAL3's influence on hyaluronan degradation could alter joint structure and function potentially disrupting normal joint movements. These associations with various proteins in disease contexts suggest the importance of HYAL3 in pathophysiological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Facilitates sperm penetration into the layer of cumulus cells surrounding the egg by digesting hyaluronic acid. Involved in induction of the acrosome reaction in the sperm. Involved in follicular atresia, the breakdown of immature ovarian follicles that are not selected to ovulate. Induces ovarian granulosa cell apoptosis, possibly via apoptotic signaling pathway involving CASP8 and CASP3 activation, and poly(ADP-ribose) polymerase (PARP) cleavage. Has no hyaluronidase activity in embryonic fibroblasts in vitro. Has no hyaluronidase activity in granulosa cells in vitro.
See full target information HYAL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37569378

2023

Amyloid Beta Peptides Lead to Mast Cell Activation in a Novel 3D Hydrogel Model.

Applications

Unspecified application

Species

Unspecified reactive species

Jingshu Liu,Sihan Liu,Li Zeng,Irene Tsilioni
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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