Anti-Hyaluronan synthase 2 antibody [4E7]

• Flow Cyt

Unknown

Flow Cytometry - Anti-Hyaluronan synthase 2 antibody [4E7] (AB140671)

Overlay histogram showing HeLa cells stained with ab140671 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140671, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hyaluronan synthase 2 antibody [4E7] (AB140671)

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Hyaluronan synthase 2 with ab140671 at 1/200 dilution followed by DAB staining.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Hyaluronan synthase 2 antibody [4E7] (AB140671)

Immunofluorescenct analysis of HeLa cells labelling Hyaluronan synthase 2 with ab140671 at 1/100 dilution (green); Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

• WB

Unknown

Western blot - Anti-Hyaluronan synthase 2 antibody [4E7] (AB140671)

All lanes:

Western blot - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution

Lane 1:

NTERA2 cell lysate

Lane 2:

HEK293 cell lysate

Predicted band size: 64 kDa

false

• WB

Unknown

Western blot - Anti-Hyaluronan synthase 2 antibody [4E7] (AB140671)

Expected MW is 37.5 kDa

All lanes:

Western blot - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution

All lanes:

Recombinant Human Hyaluronan synthase 2 protein

Predicted band size: 64 kDa

false

• WB

CiteAb

Western blot - Anti-Hyaluronan synthase 2 antibody [4E7] (AB140671)

Hyaluronan synthase 2 western blot using anti-Hyaluronan synthase 2 antibody [4E7] ab140671. Publication image and figure legend from Solier, S., Müller, S., et al., 2023, Nature, PubMed 37100912.

ab140671 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab140671 please see the product overview.

CD44 mediates the uptake of metals in inflammatory macrophages.a, ICP-MS of cellular metals in aMDM treated with an anti-CD44 antibody RG7356 during activation (n = 7 donors). b, ICP-MS of cellular metals in aMDM supplemented with HA (0.6-1 MDa) or permethylated (meth-) HA during activation (n = 6 donors). c, Western blots of hyaluronan synthases (HAS) (n = 6 donors) and ATP7A/B (n = 8 donors) in MDM. d, Fluorescence microscopy of a lysosomal copper(II) probe (Lys-Cu) in aMDM under CD44 knockdown conditions (n = 5 donors). e, Fluorescence microscopy of CTR2 and Lamp2 in aMDM (n = 4 donors). f, Fluorescence microscopy of a lysosomal copper(II) probe (Lys-Cu) in aMDM under CTR2 knockdown conditions (n = 5 donors). For d–f, Scale bars, 10 μm. For a, c, d and f two-sided Mann-Whitney test. For b, Kruskal-Wallis test with Dunn’s post-test. Box plots : boxes represent interquartile range and median, and whiskers indicate the minimum and maximum values. Each colored dot represents a distinct donor for a given panel.Source data

false