Rabbit Polyclonal HYPE antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FICD aa 50-200.
View Alternative Names
HIP13, HYPE, UNQ3041/PRO9857, FICD, Protein adenylyltransferase FICD, AMPylator FICD, De-AMPylase FICD, FIC domain-containing protein, Huntingtin yeast partner E, Huntingtin-interacting protein 13, Huntingtin-interacting protein E, HIP-13
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-HYPE antibody (AB237521)
PC-3 (Human prostate adenocarcinoma cell line) cells stained for HYPE (green) using ab237521 at 1/166 dilution in ICC/IF. Secondary antibody is an Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
- WB
Supplier Data
Western blot - Anti-HYPE antibody (AB237521)
All lanes:
Western blot - Anti-HYPE antibody (ab237521) at 1/500 dilution
Lane 1:
A549 (human lung carcinoma cell line) whole cell lysate
Lane 2:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3:
Rat kidney tissue lysate
Secondary
All lanes:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYPE antibody (AB237521)
Paraffin-embedded human small intestine tissue stained for HYPE using ab237521 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HYPE antibody (AB237521)
Paraffin-embedded human kidney tissue stained for HYPE using ab237521 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein impacts endoplasmic reticulum stress responses and protein homeostasis. It does not seem to form part of a larger complex but operates independently to regulate the degradation of specific proteins aiding in maintaining cellular balance. HYPE's actions contribute to preventing protein aggregation that could disrupt cellular function.
Pathways
HYPE participates in the unfolded protein response (UPR) and the ER-associated degradation (ERAD) pathway. It acts alongside proteins involved in protein folding and trafficking such as BiP and calnexin to ensure synthesis and processing occur efficiently. These pathways highlight HYPE’s interaction with mechanisms that manage protein quality control.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Oncology reports 51: PubMed38624012
2024
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com