AB60272

Anti-I-FABP antibody

Name: Anti-I-FABP antibody (ab60272) | Abcam
Brand: Abcam Limited
SKU: AB60272
Price: 525 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(5 Publications)

Goat Polyclonal I-FABP antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Human, Cat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human FABP2 aa 100 to C-terminus.

View Alternative Names

FABPI, FABP2, Fatty acid-binding protein 2, Intestinal-type fatty acid-binding protein, I-FABP

3 Images

Flow Cytometry - Anti-I-FABP antibody (AB60272)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-I-FABP antibody (AB60272)

ab60272 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-I-FABP antibody (AB60272)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-I-FABP antibody (AB60272)

ab60272 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Supplier Data

Western blot - Anti-I-FABP antibody (AB60272)

Detected by chemiluminescence.

Lane 1:

Western blot - Anti-I-FABP antibody (ab60272) at 0.001 µg/mL

Lane 2:

Western blot - Anti-I-FABP antibody (ab60272) at 0.003 µg/mL

Lane 1:

Human Duodenum lysate in RIPA buffer at 35 µg

Lane 2:

Ileum lysate in RIPA buffer at 35 µg

Predicted band size: 15 kDa

Observed band size: 15-16 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Cat

Applications

Flow Cyt, ELISA, ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human FABP2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P12104

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Reactivity data

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detection limit dilution 1:64000.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.003 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-3 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "Antibody detection limit dilution 1:64000.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

See full target information FABP2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 11: PubMed33375218

2020

Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport.

Applications

Unspecified application

Species

Unspecified reactive species

Min Tian,Jiaming Chen,Zhihui Wu,Hanqing Song,Fei Yang,Chang Cui,Fang Chen,Shihai Zhang,Wutai Guan

Frontiers in physiology 11:596457 PubMed33391016

2020

It's All About Timing: Early Feeding Promotes Intestinal Maturation by Shifting the Ratios of Specialized Epithelial Cells in Chicks.

Applications

Unspecified application

Species

Unspecified reactive species

Naama Reicher,Tal Melkman-Zehavi,Jonathan Dayan,Zehava Uni

Cellular and molecular gastroenterology and hepato 10:623-637 PubMed32474164

2020

The EXTrauterine Environment for Neonatal Development Supports Normal Intestinal Maturation and Development.

Applications

Unspecified application

Species

Unspecified reactive species

Heron D Baumgarten,Christina M Wright,Avery C Rossidis,Kendall M Lawrence,Aimee G Kim,Ali Y Mejaddam,Patrick E McGovern,Melissa N Orr,Barbara E Coons,Zoya Butt,Haiying Li,Grace Hwang,Antoneta Radu,Lauren J Brown,Ronald C Rubenstein,William H Peranteau,Marcus Davey,Robert O Heuckeroth,Alan W Flake

PloS one 13:e0200674 PubMed30138372

2018

Epithelial restitution defect in neonatal jejunum is rescued by juvenile mucosal homogenate in a pig model of intestinal ischemic injury and repair.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda L Ziegler,Tiffany A Pridgen,Juliana K Mills,Liara M Gonzalez,Laurianne Van Landeghem,Jack Odle,Anthony T Blikslager

PloS one 7:e51391 PubMed23236492

2012

Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis.

Applications

WB, ICC/IF

Species

Cat, Cat

Gudrun Treutlein,Roswitha Dorsch,Kerstin N Euler,Stefanie M Hauck,Barbara Amann,Katrin Hartmann,Cornelia A Deeg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-I-FABP antibody

Flow Cytometry - Anti-I-FABP antibody (AB60272)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-I-FABP antibody (AB60272)

Western blot - Anti-I-FABP antibody (AB60272)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise