AB230341

Anti-IB-1 antibody

Name: Anti-IB-1 antibody (ab230341) | Abcam
Brand: Abcam Limited
SKU: AB230341
Price: 585 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal IB-1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MAPK8IP1 aa 450-500.

View Alternative Names

IB1, JIP1, PRKM8IP, MAPK8IP1, C-Jun-amino-terminal kinase-interacting protein 1, JIP-1, JNK-interacting protein 1, Islet-brain 1, JNK MAP kinase scaffold protein 1, Mitogen-activated protein kinase 8-interacting protein 1, IB-1

4 Images

Flow Cytometry (Intracellular) - Anti-IB-1 antibody (AB230341)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IB-1 antibody (AB230341)

Intracellular flow cytometric analysis ofMCF7 (human breast adenocarcinoma cell line) cells labeling IB-1 with ab230341 at 1/10 dilution (green) compared with anegative control control (blue).

FITC-conjugated goat-anti-rabbit secondary antibodies were used.

Immunocytochemistry/ Immunofluorescence - Anti-IB-1 antibody (AB230341)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IB-1 antibody (AB230341)

MCF7 (human breast adenocarcinoma cell line) cells stained for IB-1 (green) using ab230341 at 1/10 dilution followed by Alexa Fluor^® 488-congugated Goat Anti-Rabbit IgG in ICC/IF.

The nuclear counter stain is DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IB-1 antibody (AB230341)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IB-1 antibody (AB230341)

Formalin-fixed, paraffin-embedded human kidney tissue stained for IB-1 with ab230341 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-IB-1 antibody (AB230341)

All lanes:

Western blot - Anti-IB-1 antibody (ab230341) at 1/1000 dilution

All lanes:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 35 µg

Predicted band size: 78 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human MAPK8IP1 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q9UQF2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10 - 1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response. Acts as a scaffold protein that coordinates with SH3RF1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the activation of MAPK8/JNK1 and differentiation of CD8(+) T-cells.

See full target information MAPK8IP1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

European urology open science 79:102-110 PubMed40822998

2025

Expression of PD-L1, TIGIT, and CD155, and Human Papillomavirus Status in Patients with Advanced Penile Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Ulvskog,Peter Kirrander,Erik K Persson,Gabriella Lillsunde-Larsson,Luiza Dorofte,Tania Franceschini,Michelangelo Fiorentino,Sabina Davidsson

Journal of oral & facial pain and headache 39:196-203 PubMed40129438

2025

Loss of SCRG1 in chondrocytes inhibits osteoarthritis by promoting autophagy activity in the temporomandibular joint through inhibition of neurokine receptors.

Applications

Unspecified application

Species

Unspecified reactive species

JiaJun Zhang,LePing Yuan,YanYan Zhang,HaoYang Jin,YeKe Zhao,XiaoKe Zeng,YanHui Zou,KeYu Wang,Xin Nie

Allergologia et immunopathologia 53:160-168 PubMed40088033

2025

DUOX1 inhibits the progression of rheumatoid arthritis by regulating the NF-κB pathway in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Xuan,Dandan Feng,Fuyong Qiang,Yonghui Xia

Allergologia et immunopathologia 52:73-79 PubMed39278854

2024

Shengmai Powder regulates alveolar macrophage PPAR-γ and improves the chronic inflammatory state of chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Liu,Zongwei Liu,Xunxun Ma,Shengjie Wang,Jie Lin,Xiuyan Shi,Xiaoyong Xu

International journal of rheumatic diseases 26:80-87 PubMed36195975

2022

Casticin promotes osteogenic differentiation of bone marrow stromal cells and improves osteoporosis in rats by regulating nuclear factor-κB/mitogen-activated protein kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhang,Xuejia Li,Jianmin Li,Wanxin Liu,Ying Yu,Shuqiang Wang,Xiaojian Ye

Frontiers in pharmacology 11:616813 PubMed33574759

2021

AMPK/SIRT1 Pathway is Involved in Arctigenin-Mediated Protective Effects Against Myocardial Ischemia-Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Yin Liu,Yi Zhou,Tao Chen,Jing-Chao Lei,Xue-Jun Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IB-1 antibody

Flow Cytometry (Intracellular) - Anti-IB-1 antibody (AB230341)

Immunocytochemistry/ Immunofluorescence - Anti-IB-1 antibody (AB230341)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IB-1 antibody (AB230341)

Western blot - Anti-IB-1 antibody (AB230341)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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