Anti-IB-1 antibody [EPR30423-508]

• WB

Lab

Western blot - Anti-IB-1 antibody [EPR30423-508] (AB325525)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Jurkat, A20, EL4

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 29540552).

The identity of the lower MW bands at approximately 60 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IB-1 antibody [EPR30423-508] (ab325525) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 40 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg

Lane 4:

A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 40 µg

Lane 5:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 110 kDa,120 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-IB-1 antibody [EPR30423-508] (AB325525)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : testis, kidney, lung

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 29540552).

The identity of the lower MW bands at approximately 60 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IB-1 antibody [EPR30423-508] (ab325525) at 1/1000 dilution

Lane 1:

Human spinal cord tissue lysate at 40 µg

Lane 2:

Human cerebral cortex tissue lysate at 40 µg

Lane 3:

Human testis tissue lysate at 40 µg

Lane 4:

Human kidney tissue lysate at 40 µg

Lane 5:

Human lung tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 110 kDa,120 kDa,36 kDa

false

Exposure time: 48s

• WB

Lab

Western blot - Anti-IB-1 antibody [EPR30423-508] (AB325525)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver, testis, spleen

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 29540552).

The identity of the lower MW bands at approximately 60 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IB-1 antibody [EPR30423-508] (ab325525) at 1/1000 dilution

Lane 1:

Mouse brain fresh tissue lysate at 20 µg

Lane 2:

Mouse liver fresh tissue lysate at 20 µg

Lane 3:

Mouse testis fresh tissue lysate at 20 µg

Lane 4:

Mouse spleen fresh tissue lysate at 20 µg

Lane 5:

Rat brain fresh tissue lysate at 20 µg

Lane 6:

Rat liver fresh tissue lysate at 20 µg

Lane 7:

Rat testis fresh tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 110 kDa,120 kDa,36 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Anti-IB-1 antibody [EPR30423-508] (AB325525)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human IB-2 or IB-3.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(110,120KDa); anti- H3 antibody (ab176842)(1 : 1000)(15 KDa)..

All lanes:

Western blot - Anti-IB-1 antibody [EPR30423-508] (ab325525) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 10 µg

Lane 2:

293T cells transfected with a human IB-1expression vector containing a his-tag, whole cell lysate at 3 µg

Lane 3:

293T cells transfected with a human IB-2 expression vector containing a his-tag, whole cell lysate at 10 µg

Lane 4:

293T cells transfected with a human IB-3 expression vector containing a his-tag, whole cell lysate at 8 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 110 kDa,120 kDa,15 kDa

false

Exposure time: 6s

• Dot

Lab

Dot Blot - Anti-IB-1 antibody [EPR30423-508] (AB325525)

Dot blot analysis of IB-1 using ab325525 at 1 : 1000 (0.524 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : 293T cells transfected with an empty vector containing a his-tag, whole cell lysate

Lane 2 : 293T cells transfected with a human IB-1expression vector containing a his-tag, whole cell lysate

Lane 3 : 293T cells transfected with a human IB-2 expression vector containing a his-tag, whole cell lysate

Lane 4 : 293T cells transfected with a human IB-3 expression vector containing a his-tag, whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human IB-2 or IB-3.

All lanes:

Dot Blot - Anti-IB-1 antibody [EPR30423-508] (ab325525) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate

Lane 2:

293T cells transfected with a human IB-1expression vector containing a his-tag, whole cell lysate

Lane 3:

293T cells transfected with a human IB-2 expression vector containing a his-tag, whole cell lysate

Lane 4:

293T cells transfected with a human IB-3 expression vector containing a his-tag, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s