Rabbit Polyclonal IB-2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAPK8IP2 aa 500 to C-terminus.
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Publications
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- International journal of molecular sciences 21:2020Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions.
- PLoS pathogens 14:e10069422018miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMalak Haidar et. al.PubMed 29570727
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAPK8IP2 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13387
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Target data
Function
The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity).
Alternative names
IB2, JIP2, PRKM8IPL, MAPK8IP2, C-Jun-amino-terminal kinase-interacting protein 2, JIP-2, JNK-interacting protein 2, Islet-brain-2, JNK MAP kinase scaffold protein 2, Mitogen-activated protein kinase 8-interacting protein 2, IB-2
Recommended products
Rabbit Polyclonal IB-2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAPK8IP2 aa 500 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAPK8IP2 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13387
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-IB-2 antibody (ab154090)
7.5% SDS PAGE
All lanes: Western blot - Anti-IB-2 antibody (ab154090) at 1/1000 dilution
All lanes: Molt4 whole cell lysate at 30 µg
Predicted band size: 87 kDa
Western blot - Anti-IB-2 antibody (ab154090)
7.5% SDS PAGE
All lanes: Western blot - Anti-IB-2 antibody (ab154090) at 1/1000 dilution
All lanes: mouse brain whole cell lysate at 50 µg
Predicted band size: 87 kDa
Immunocytochemistry/ Immunofluorescence - Anti-IB-2 antibody (ab154090)
Immunofluorescence analysis of methanol-fixed HeLa cells labeling IB-2 with ab154090 at 1/200 dilution. The image in the lower panel is costained with Hoechst 33342.
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