Goat Polyclonal Iba1 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 150 publications.
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- Nature communications 14:49652023Astroglial Hmgb1 regulates postnatal astrocyte morphogenesis and cerebrovascular maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoises Freitas-Andrade,Cesar H Comin,Peter Van Dyken,Julie Ouellette,Joanna Raman-Nair,Nicole Blakeley,Qing Yan Liu,Sonia Leclerc,Youlian Pan,Ziying Liu,Micaël Carrier,Karan Thakur,Alexandre Savard,Gareth M Rurak,Marie-Ève Tremblay,Natalina Salmaso,Luciano da F Costa,Gianfilippo Coppola,Baptiste LacostePubMed 37587100
- Life science alliance 6:2023Neuroprotective effects of hepatoma-derived growth factor in models of Huntington's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKerstin Voelkl,Sara Gutiérrez-Ángel,Sophie Keeling,Seda Koyuncu,Miguel da Silva Padilha,Dennis Feigenbutz,Thomas Arzberger,David Vilchez,Rüdiger Klein,Irina DudanovaPubMed 37580082
- Frontiers in aging neuroscience 15:11407082023Leukotriene signaling as molecular correlate for cognitive heterogeneity in aging: an exploratory study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeike Mrowetz,Mohamed H Kotob,Jennifer Forster,Iren Aydin,Michael Stefan Unger,Jana Lubec,Ahmed M Hussein,Jovana Malikovic,Daniel Daba Feyissa,Volker Korz,Harald Höger,Gert Lubec,Ludwig AignerPubMed 37600518
- Nature biotechnology 42:892-9042023Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAtharva Sahasrabudhe,Laura E Rupprecht,Sirma Orguc,Tural Khudiyev,Tomo Tanaka,Joanna Sands,Weikun Zhu,Anthony Tabet,Marie Manthey,Harrison Allen,Gabriel Loke,Marc-Joseph Antonini,Dekel Rosenfeld,Jimin Park,Indie C Garwood,Wei Yan,Farnaz Niroui,Yoel Fink,Anantha Chandrakasan,Diego V Bohórquez,Polina AnikeevaPubMed 37349522
- Frontiers in physiology 14:11514952023Pericyte-derived cells participate in optic nerve scar formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Preishuber-Pflügl,Daniela Mayr,Veronika Altinger,Susanne M Brunner,Andreas Koller,Christian Runge,Anja-Maria Ladek,Markus Lenzhofer,Francisco J Rivera,Herbert Tempfer,Ludwig Aigner,Herbert A Reitsamer,Andrea TrostPubMed 37143930
- iScience 26:1062772023Administration of prosaposin-derived neurotrophic factor to neural tube defects facilitates regeneration and restores neurological functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSakirul Khan,Akihide Takeuchi,Hiroaki Nabeka,Farzana Khan,Tetsuya Shimokawa,Sota Takanezawa,Takashi Saitou,Takeshi Imamura,Tetsuya Tachibana,Akira Nishizono,Fumihiko Hamada,Seiji MatsudaPubMed 37153447
- The Journal of neuroscience : the official journal of the Society for Neuroscience 43:1814-18292023Activation of PPARα Exhibits Therapeutic Efficacy in a Mouse Model of Juvenile Neuronal Ceroid Lipofuscinosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMalabendu Jana,Debashis Dutta,Jit Poddar,Kalipada PahanPubMed 36697260
- Frontiers in aging neuroscience 14:10254022022Hypocretin/orexin influences chronic sleep disruption injury in the hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHenry Nick,Polina Fenik,Yan Zhu,Sigrid VeaseyPubMed 36275002
- Visual neuroscience 39:E0052022Glial cell response to constant low light exposure in rat retina.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManuel G Bruera,María M Benedetto,Mario E Guido,Alicia L Degano,María A ContinPubMed 36164752
- PloS one 17:e02691402022Acyloxyacyl hydrolase regulates microglia-mediated pelvic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAfrida Rahman-Enyart,Ryan E Yaggie,Justin L Bollinger,Constadina Arvanitis,Deborah R Winter,Anthony J Schaeffer,David J KlumppPubMed 35980963
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Rat | Tested |
| Chinese hamster | Predicted |
| Common marmoset | Predicted |
| Dog | Predicted |
| Macaque monkey | Predicted |
| Pig | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Macaque monkey, Chinese hamster, Common marmoset | Dilution info - | Notes - |
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Target data
Function
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play an role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation (By similarity).
Alternative names
Iba1, Mrf1, Aif1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, MRF-1, Microglia response factor
Recommended products
Goat Polyclonal Iba1 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 150 publications.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
If looking for a monoclonal anti-Iba1 alternative we can recommend our RabMAb ab178846. This has the same immunogen as ab5076.
In recent testing in IHC-P this antibody only worked in Rat tissue, therefore we have removed Human and mouse as a "does not react with" species. However, feedback from our customers and publications suggest that this antibody recognizes human and mouse. Nevertheless, we cannot guarantee this antibody in these species.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.
- Biological function summary
Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.
- Pathways
Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.
- Associated diseases and disorders
Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab107159)
IHC-P image of Iba1 staining on marmoset (common) kidney sections using ab107159 (1:5000). The image shows macrophage infiltration in the cortex. The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 16 hours. The secondary antibody was rabbit polyclonal to sheep IgG conjugated to biotin at 1:200.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab107159)
IHC image of Iba1 staining in a section of formalin-fixed paraffin-embedded normal rat brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab107159, 5ug/ml, for 15 mins at room temperature. An HRP-conjugated donkey anti-goat IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab107159)
ab107159 staining Iba1 in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted HRP-conjugated anti-goat IgG polyclonal was used as the secondary antibody. Kupffer cells are positive for Iba-1.
This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab107159)
IHC-P image of Iba1 staining on rat spleen sections using ab107159 (1:2000). The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours. The secondary antibody was goat polyclonal to anti-sheep conjugated to biotin at 1:250.
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