Rabbit Polyclonal Iba1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human AIF1.
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- Nutrients 15:2023Effect of Oleoylethanolamide-Based Dietary Supplement on Systemic Inflammation in the Development of Alimentary-Induced Obesity in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDarya Ivashkevich,Arina Ponomarenko,Igor Manzhulo,Ruslan Sultanov,Inessa DyuizenPubMed 37892420
- International journal of molecular sciences 24:2023Synaptamide Ameliorates Hippocampal Neurodegeneration and Glial Activation in Mice with Traumatic Brain Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Tyrtyshnaia,Olga Manzhulo,Igor ManzhuloPubMed 37373162
- Frontiers in molecular biosciences 9:8876782022Lipid nanoparticles for antisense oligonucleotide gene interference into brain border-associated macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMacarena Calero,Lara H Moleiro,Aline Sayd,Yeray Dorca,Lluis Miquel-Rio,Verónica Paz,Javier Robledo-Montaña,Eduardo Enciso,Fernando Acción,Diego Herráez-Aguilar,Thomas Hellweg,Luis Sánchez,Analía Bortolozzi,Juan C Leza,Borja García-Bueno,Francisco MonroyPubMed 36406277
- Inflammopharmacology 30:1745-17582022Diapocynin neuroprotective effects in 3-nitropropionic acid Huntington's disease model in rats: emphasis on Sirt1/Nrf2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeam W Ibrahim,Nora O Abdel RasheedPubMed 35639233
- Frontiers in immunology 13:8115252022TNFAIP3 Reduction-of-Function Drives Female Infertility and CNS Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan W Zammit,Joseph McDowell,Joanna Warren,Walter Muskovic,Joanne Gamble,Yan-Chuan Shi,Dominik Kaczorowski,Chia-Ling Chan,Joseph Powell,Chris Ormandy,David Brown,Samantha R Oakes,Shane T GreyPubMed 35464428
- Journal of cellular and molecular medicine 26:2633-26452022Analysis of necroptosis and its association with pyroptosis in organ damage in experimental pulmonary arterial hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIzabela Jarabicová,Csaba Horváth,Eva Veľasová,Lenka Bies Piváčková,Jana Vetešková,Ján Klimas,Peter Křenek,Adriana AdameováPubMed 35393789
- Inflammopharmacology 30:919-9342022Neuroprotective effect of liraglutide in an experimental mouse model of multiple sclerosis: role of AMPK/SIRT1 signaling and NLRP3 inflammasome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReham A Ammar,Ahmed F Mohamed,Mohamed M Kamal,Marwa M Safar,Noha F AbdelkaderPubMed 35364735
- International journal of molecular sciences 22:2021Synaptamide Improves Cognitive Functions and Neuronal Plasticity in Neuropathic Pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Tyrtyshnaia,Anatoly Bondar,Sophia Konovalova,Igor ManzhuloPubMed 34884587
- International journal of molecular sciences 22:2021Anti-Inflammatory Activity of -Docosahexaenoylethanolamine and -Eicosapentaenoylethanolamine in a Mouse Model of Lipopolysaccharide-Induced Neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Tyrtyshnaia,Sophia Konovalova,Anatoly Bondar,Ekaterina Ermolenko,Ruslan Sultanov,Igor ManzhuloPubMed 34639071
- The Journal of neuroscience : the official journal of the Society for Neuroscience 41:9286-93072021DNA Repair Inhibition Leads to Active Export of Repetitive Sequences to the Cytoplasm Triggering an Inflammatory Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Song,Jacqueline T M Aw,Fulin Ma,Ming Fung Cheung,Danny Leung,Karl HerrupPubMed 34593604
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human AIF1. The exact immunogen used to generate this antibody is proprietary information. Database link P55008
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes We recommend blocking in 3-5% milk. Blocking with BSA gives high background. |
Species Rat | Dilution info 1/500.00000 - 1/1000.00000 | Notes We recommend blocking in 3-5% milk. Blocking with BSA gives high background. |
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes We recommend blocking in 3-5% milk. Blocking with BSA gives high background. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Alternative names
G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1
Recommended products
Rabbit Polyclonal Iba1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human AIF1.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human AIF1. The exact immunogen used to generate this antibody is proprietary information. Database link P55008
- Purification technique
Affinity purification Immunogen
- Specificity
In WB, we recommend blocking in milk. Blocking with BSA gives high background. If looking for a monoclonal anti-Iba1 alternative we can recommend our RabMAb ab178846
- Reconstitution
Reconstitute in 200 µL of water
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.
- Biological function summary
Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.
- Pathways
Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.
- Associated diseases and disorders
Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
IHC image of Iba1 staining in normal mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
IHC image of Iba1 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
IHC image of Iba1 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
ab108539, at 1/100 dilution, staining Iba1 in formalin-fixed, paraffin-embedded Human fetal lymphocytes by Immunohistochemistry.
Western blot - Anti-Iba1 antibody (ab108539)
Lanes 1-14: Blocked in 3% milk for 1 hour (RT). Lanes 15-16: Blocked in 5% BSA for 1 hour (RT).
Abcam recommends blocking in milk for cleaner blots with reduced background, in comparison to BSA.
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab108539 (anti-Iba1 antibody; 1/1000) for 18 hours at 4°C. Antibody binding was detected using HRP-labelled anti-Rabbit IgG for 1 hour at room temperature and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Iba1 antibody (ab108539) at 1/1000 dilution
Lane 1: Human Iba1 recombinant protein at 0.1 µg
Lane 2: HEK293 whole cell lysate at 20 µg
Lane 3: A431 whole cell lysate at 20 µg
Lane 4: NIH3T3 whole cell lysate at 30 µg
Lane 5: Human spleen tissue lysate at 20 µg
Lane 6: Mouse spleen tissue lysate at 30 µg
Lane 7: Rat spleen tissue lysate at 30 µg
Lane 8: U937 whole cell lysate at 30 µg
Lane 9: MOLT4 whole cell lysate at 20 µg
Lane 10: THP1 whole cell lysate at 30 µg
Lane 11: THP1 whole cell lysate, PMA treated at 30 µg
Lane 12: Raw 264.7 whole cell lysate at 30 µg
Lane 13: C6 whole cell lysate at 30 µg
Lane 14: NR8383 whole cell lysate at 30 µg
Lane 15: NIH3T3 whole cell lysate - BLOCKED IN 5% BSA at 30 µg
Lane 16: Human spleen tissue lysate - BLOCKED IN 5% BSA at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Exposure time: 3min
Western blot - Anti-Iba1 antibody (ab108539)
All lanes: Western blot - Anti-Iba1 antibody (ab108539) at 1/500 dilution
All lanes: Rat brain lysate
Predicted band size: 16 kDa
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