Anti-Iba1 antibody is a goat polyclonal antibody that is used to detect Iba1 in IHC-P, Western blot. Suitable for Human, Rat samples.
- Cited in over 1350 publications
- Tried and trusted by researchers since 2003
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- Brain pathology (Zurich, Switzerland) :e133052024hnRNP A1, hnRNP A2B1, and hnRNP K are dysregulated in tauopathies, but do not colocalize with tau pathology.Applications:IHC-PReactive species:HumanTomas Kavanagh,Kaleah Balcomb,Diba Ahmadi Rastegar,Guinevere F Lourenco,Thomas Wisniewski,Glenda Halliday,Eleanor DrummondPubMed 39354671
- Aging and disease 15:1384-13972024Thioredoxin1 Binding Metastasis-Associated Lung Adenocarcinoma Transcript 1 Attenuates Inflammation and Apoptosis after Intracerebral Hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRu Chen,Qi Xie,Lexing Xie,Jiacheng Huang,Linlin Hu,Hui Lu,Peixia Shi,Qian He,Qin Zhang,Changxiong Gong,Shuang Zhang,Bingqiao Wang,Guoqiang Yang,Qingwu YangPubMed 37196136
- Aging and disease 15:390-4072024SARM1 Promotes Neurodegeneration and Memory Impairment in Mouse Models of Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuemeng Miao,Qian Wu,Siyu Du,Ludan Xiang,Siyao Zhou,Junzhe Zhu,Zirun Chen,Hui Wang,Xuyi Pan,Yiren Fan,Lihan Zhang,Jingkang Qian,Yuxuan Xing,Yiyang Xie,Lixin Hu,Haiyun Xu,Wei Wang,Ying Wang,Zhihui HuangPubMed 37307837
- JCI insight 9:2024Neuronal deletion of the circadian clock gene Bmal1 induces cell-autonomous dopaminergic neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael F Kanan,Patrick W Sheehan,Jessica N Haines,Pedro G Gomez,Adya Dhuler,Collin J Nadarajah,Zachary M Wargel,Brittany M Freeberg,Hemanth R Nelvagal,Mariko Izumo,Joseph S Takahashi,Jonathan D Cooper,Albert A Davis,Erik S MusiekPubMed 38032732
- Translational stroke research :2023Inhibition of USP30 Promotes Mitophagy by Regulating Ubiquitination of MFN2 by Parkin to Attenuate Early Brain Injury After SAH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Liu,Chenbei Yao,Bin Sheng,Simin Zhi,Xiangxin Chen,Pengfei Ding,Jiatong Zhang,Zhennan Tao,Wei Li,Zong Zhuang,Jiannan Mao,Zheng Peng,Huiying Yan,Wei JinPubMed 38147294
- eLife 12:2023Microglia facilitate and stabilize the response to general anesthesia via modulating the neuronal network in a brain region-specific manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang He,Taohui Liu,Quansheng He,Wei Ke,Xiaoyu Li,Jinjin Du,Suixin Deng,Zhenfeng Shu,Jialin Wu,Baozhi Yang,Yuqing Wang,Ying Mao,Yanxia Rao,Yousheng Shu,Bo PengPubMed 38131301
- Journal of inflammation (London, England) 20:432023Circadian dependency of microglial heme oxygenase-1 expression and inflammation determine neuronal injury in hemorrhagic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuise Henrich,Iva Kiessling,Matti Steimer,Sibylle Frase,Sandra Kaiser,Nils SchallnerPubMed 38104143
- International journal of molecular sciences 24:2023Trimethylamine N-Oxide as a Mediator Linking Peripheral to Central Inflammation: An In Vitro Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManuel H Janeiro,Maite Solas,Josune Orbe,Jose A Rodríguez,Leyre Sanchez de Muniain,Paula Escalada,Ping K Yip,Maria J RamirezPubMed 38139384
- Nature communications 14:75522023INPP5D regulates inflammasome activation in human microglia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVicky Chou,Richard V Pearse,Aimee J Aylward,Nancy Ashour,Mariko Taga,Gizem Terzioglu,Masashi Fujita,Seeley B Fancher,Alina Sigalov,Courtney R Benoit,Hyo Lee,Matti Lam,Nicholas T Seyfried,David A Bennett,Philip L De Jager,Vilas Menon,Tracy L Young-PearsePubMed 38016942
- Science advances 9:eadk10502023Biochemically functionalized probes for cell-type-specific targeting and recording in the brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnqi Zhang,Theodore J Zwang,Charles M LieberPubMed 38019917
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human AIF1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P55008
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Not recommended | Tested |
| Macaque monkey | Not recommended | Predicted |
| Pig | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee. |
Species Pig | Dilution info - | Notes - |
Species Macaque monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-3 µg/mL | Notes A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background. |
Species Human | Dilution info 1-3 µg/mL | Notes A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Macaque monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Additional Targets
Aif1
Alternative names
G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1
Recommended products
Anti-Iba1 antibody is a goat polyclonal antibody that is used to detect Iba1 in IHC-P, Western blot. Suitable for Human, Rat samples.
- Cited in over 1350 publications
- Tried and trusted by researchers since 2003
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human AIF1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P55008
- Purification technique
- Affinity purification Immunogen
- Specificity
ab5076 is expected to recognize the isoforms represented by NP_001614 and NP_116573 but not NP_004838.
Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Find all reagents to label astrocytes in our "Microglia markers guide".
Anti-Iba1 antibody (ab5076) is a Goat Polyclonal antibody and is validated for use in IHC-P, WB in human, mouse, rat samples.
Anti-Iba1 antibody (ab5076) has been cited over 1354 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-Iba1 antibody (ab5076) has high sensitivity and specificity.
Anti-Iba1 antibody (ab5076) has 100 independent reviews from customers.
Anti-Iba1 antibody (ab5076) specifically detects Iba1 (UniProt ID: P55008; Molecular weight: 17kDa) and is sold in 100 ug selling sizes.
Iba1 (Ionized calcium-binding adapter molecule 1; allograft inflammatory factor 1, AIF1) is a prominent microglial marker extensively used in neuroscience research. Its expression increases in various neurodegenerative conditions, including Alzheimer's disease (AD), indicating microglial activation and neuroinflammation. Researchers utilize Iba1 in stainings to assess microglial response and activity in different models, making it essential for understanding inflammatory processes in the brain. Changes in Iba1 expression provide valuable insights into neuroinflammation, highlighting its importance in studying diseases like AD.
The Iba1 antibody (ab5076) is commonly used as a marker of microglia activation in staining and immunohistochemistry, given that ionized calcium binding adaptor molecule 1 (Iba1) is a microglia/macrophage-specific calcium-binding protein with actin-bundling activity that participates in membrane ruffling and phagocytosis in activated microglia.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Iba1 also known as allograft inflammatory factor 1 (AIF1) is a protein with a molecular weight of approximately 17 kDa. It is widely expressed in microglia which are the resident immune cells of the central nervous system (CNS). Iba1 plays a mechanical role in actin-binding and modulating cytoskeleton dynamics which affects cell shape and motility. It is also found in other myeloid-derived cells including macrophages and monocytes where it influences cellular processes linked to inflammation and immune responses.
- Biological function summary
The involvement of Iba1 in actin polymerization impacts microglia's ability to perform phagocytosis and cytokine production. Iba1 does not appear to be part of any large protein complex but interacts directly with actin filaments to facilitate cytoskeletal reorganization. This function enables the cells to move and respond efficiently to tissue damage or pathogens in the CNS. It plays an important role in supporting cellular changes associated with immune surveillance and neuroinflammatory responses.
- Pathways
Microglial activation relies heavily on Iba1 for executing acts linked with neuroinflammation. Iba1 engages in the NF-kB signaling pathway significant in managing inflammatory responses. It interacts with proteins such as MyD88 and IRAK4 which aid in transmitting signals from toll-like receptors. These pathways enable changes in gene expression critical for microglial response to various stimuli. Furthermore Iba1 also contributes to the PI3K/Akt pathway where it influences cell survival and proliferation interacting with proteins like Akt1 and PTEN.
- Associated diseases and disorders
Iba1 has implications in neurodegenerative diseases notably Alzheimer's disease and multiple sclerosis. In Alzheimer's disease increased expression of Iba1 is observed in activated microglia around amyloid plaques suggesting a role in plaque clearance and neuroinflammation. The protein relates to amyloid precursor protein (APP) as microglia respond to abnormal accumulations. In multiple sclerosis Iba1 expression marks activated microglia involved in demyelination processes where it associates with proteins like myelin basic protein (MBP) highlighting its role in the disease's pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)
Iba1 immunohistochemistry staining of human spleen using goat anti-Iba1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Iba1 with ab5076 at 8µg/ml.
Heat induced antigen retrieval with citrate buffer Ph 6 HRP-staining.
Western blot - Anti-Iba1 antibody (ab5076)
Detected by chemiluminescence.
All lanes: Western blot - Anti-Iba1 antibody (ab5076) at 1 µg/mL
Lane 1: Human lymph node tissue lysate in RIPA buffer at 35 µg
Lane 2: Rat brain tissue lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 16 kDa
Image from Iris Margalit Trutzer et al, ACTA neuropathologica communications, 7, 40, Fig. 7; doi: 10.1186/s40478-019-0684-8Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lateral prefrontal cortex tissue labeling iba1 labeled microglia (labeled with black arrows) within the cortex using ab5076 at 1/1000 dilution. (Cropped image).
Sections were rinsed in 0.01 m PBS, pH 7.4, followed by 10% serum matching the species of the secondary antibody, 5% bovine serum albumin, and 0.1% Triton X-100 in 0.01 m PBS blocking solution for 1 h and incubated for 2 days at 4 °C in primary antibody. The sections were rinsed in PBS and incubated overnight at 4 °C with a horse anti-Goat IgG (Biotinylated) secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)
Iba1 immunohistochemistry staining of human spleen using goat anti-Iba1 antibody
Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Iba1 with no primary antibody.
Heat induced antigen retrieval with citrate buffer Ph 6 HRP-staining.
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