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Goat Polyclonal Iba1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 1353 publications. Immunogen corresponding to Synthetic Peptide within Human AIF1 aa 100 to C-terminus.
Alternative names=Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, IBA1, G1, AIF1
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
Synthetic Peptide within Human AIF1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link: P55008
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Not recommended | Tested |
| Macaque monkey | Not recommended | Predicted |
| Pig | Not recommended | Predicted |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee. |
Species Pig | Dilution info - | Notes - |
Species Macaque monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-3 µg/mL | Notes A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background. |
Species Human | Dilution info 1-3 µg/mL | Notes A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Macaque monkey | Dilution info - | Notes - |
Recommended products
Goat Polyclonal Iba1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 1353 publications. Immunogen corresponding to Synthetic Peptide within Human AIF1 aa 100 to C-terminus.
Alternative names=Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, IBA1, G1, AIF1
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
Synthetic Peptide within Human AIF1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link: P55008
- Purification technique
Affinity purification Immunogen
- Specificity
ab5076 is expected to recognize the isoforms represented by NP_001614 and NP_116573 but not NP_004838.
Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
The Iba1 antibody (ab5076) is commonly used as a marker of microglia activation in staining and immunohistochemistry, given that ionized calcium binding adaptor molecule 1 (Iba1) is a microglia/macrophage-specific calcium-binding protein with actin-bundling activity that participates in membrane ruffling and phagocytosis in activated microglia.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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