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Chicken Recombinant Monoclonal Iba1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (AB318305), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (AB318305), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (AB318305), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (AB318305), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (AB318305), expandable thumbnail

Key facts

Isotype
IgY
Host species
Chicken
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIHC-Fr
Human
Tested
Tested
Expected
Mouse
Tested
Expected
Tested
Rat
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
5 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Additional Targets

Aif1

Alternative names

G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1

Recommended products

Chicken Recombinant Monoclonal Iba1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgY
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16588
Purification technique
Affinity purification Thiophilic Resin
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab318305 is the carrier-free version of Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302.

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Iba1 antibody [EPR16588] ab178846). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.

Biological function summary

Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.

Pathways

Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.

Associated diseases and disorders

Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305)

    This data was developed using Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA, 0.2% Triton X-100 permeabilized frozen Rat liver (perfused-fixed) tissue labelling Iba1 with Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302 at 1/50 dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173) at 1/1000 dilution (Green).

    Confocal image showing positive staining on Rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 at 1/1000.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

  • Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305)

    This data was developed using Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (perfused-fixed) tissue labelling Iba1 with Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302 at 1/50 dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173) at 1/1000 dilution (Green).

    Confocal image showing positive staining on Rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 at 1/1000.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

  • Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305)

    This data was developed using Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA, 0.2% Tritin X-100 permeabilized frozen Mouse liver tissue labelling Iba1 with Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302 at 1/50 dilution Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173) at 1/1000 dilution (Green)

    Secondary antibody control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173) at 1/1000 dilution

    Confocal image showing positive staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

  • Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305)

    This data was developed using Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (perfused-fixed) tissue labelling Iba1 with Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302 at 1/50 dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173) at 1/1000 dilution (Green).

    Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 at 1/1000.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305)

    Immunofluorescence staining of Iba1 in a section of formalin-fixed paraffin-embedded mouse brain. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab318305 at 1 μg/ml, and then incubated for 1 hour with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305)

    ab318305 staining Iba1 in THP-1 (positive) and HeLa (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab318305 at 5 µg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318305)

    Immunofluorescence staining of Iba1 in a section of formalin-fixed paraffin-embedded human brain. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab318305 at 1 μg/ml, and then incubated for 1 hour with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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