Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric)
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(142 Publications)
Anti-Iba1 antibody [EPR16589] - Mouse IgG1 (Chimeric) (ab283319) is a mouse monoclonal antibody detecting Iba1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
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- Over 20 publications
View Alternative Names
G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, iba1, iba-1, iba 1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (AB283319)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Iba1 with ab283319 at 1/10000 (0.114 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the microglia in rat cerebrum. The section was incubated with ab283319 for 10 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (AB283319)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, Left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labelling Iba1 with ab283319 at 1/10000 dilution (0.01ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Negative control : MCF7.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (AB283319)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Iba1 with ab283319 at 1/10000 (0.114 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the microglia in human cerebrum. The section was incubated with ab283319 for 10 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (AB283319)
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Iba1 with ab283319 at 1/10000 (0.114 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the immune cells in human endometrium. The section was incubated with ab283319 for 10 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (AB283319)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 cells labelling Iba1 with ab283319 at 1/100 (11.36 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Confocal image showing cytoplasmic staining in THP-1 cells.
Negative control : MCF7
Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (AB283319)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Iba1 with ab283319 at 1/10000 (0.114 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the microglia in mouse cerebrum. The section was incubated with ab283319 for 10 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- IP
Supplier Data
Immunoprecipitation - Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (AB283319)
Iba1 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte), whole cell lysate 10 ug with ab283319 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283319 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.
Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate 10 ug
Lane 2 : ab283319 IP in THP-1 whole cell lysate
Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab283319 in THP-1 whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 32 seconds
All lanes:
Immunoprecipitation - Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (ab283319)
Predicted band size: 16 kDa
Observed band size: 16 kDa
false
- WB
Supplier Data
Western blot - Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (AB283319)
Blocking and diluting buffer : 5% NFDM/TBST
Negative control : MCF7 (HPA database)
Exposure time : 136 seconds
All lanes:
Western blot - Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (ab283319) at 1/1000 dilution
Lane 1:
THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg
Lane 2:
MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes:
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
false
Reactivity data
Product details
Find all reagents to label astrocytes in our "Microglia markers guide".
Anti-Iba1 antibody [EPR16589] - Mouse IgG1 (Chimeric) (ab283319) is a mouse monoclonal recombinant antibody validated for use in IHC-P, IP, WB, ICC/IF and Flow Cyt (Intra) in mouse, rat and human samples.
Clone EPR16589 was initially developed by Abcam using patented rabbit monoclonal antibody technology and has been engineered to generate this mouse chimeric antibody which retains the binding properties of the RabMAb parent, but has a mouse Fc region. By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Abcams high quality manufacturing and validation processes ensure Anti-Iba1 antibody [EPR16589] - Mouse IgG1 (Chimeric) (ab283319) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Iba1 antibody [EPR16589] - Mouse IgG1 (Chimeric) (ab283319) specifically detects Iba1 (AIF-1) (UniProt ID: P55008; Molecular weight: 17kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format is also available for Anti-Iba1 antibody [EPR16589] - Mouse IgG1 (Chimeric) - ab312913.
Iba1 (Ionized calcium-binding adapter molecule 1; allograft inflammatory factor 1, AIF1) is a prominent microglial marker extensively used in neuroscience research. Its expression increases in various neurodegenerative conditions, including Alzheimer's disease (AD), indicating microglial activation and neuroinflammation. Researchers utilize Iba1 in stainings to assess microglial response and activity in different models, making it essential for understanding inflammatory processes in the brain. Changes in Iba1 expression provide valuable insights into neuroinflammation, highlighting its importance in studying diseases like AD.
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab178847). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
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Shipped at conditions
Appropriate short-term storage duration
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.
Pathways
Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.
Product protocols
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Target data
Publications (142)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in pharmacology 16:1574007 PubMed41050410
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Brain and behavior 15:e70850 PubMed40923147
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Frontiers in immunology 16:1650254 PubMed40918113
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Stroke and vascular neurology : PubMed40908045
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Molecular medicine (Cambridge, Mass.) 31:284 PubMed40898058
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Materials today. Bio 34:102172 PubMed40822932
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Communications biology 8:1234 PubMed40819187
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Communications biology 8:1188 PubMed40781500
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Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70331 PubMed40731189
2025
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Cell 188:5499-5515.e20 PubMed40712576
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com