Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free (AB221933)

This data was developed using ab178680, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Paraffin-embedded sections Human cerebrum tissue labelling Iba1 with ab178680 at 1/100000 dilution, followed by a ready to use secondary LeicaDS9800 (Bond^TM Polymer Refine Detection). Counter stained with Haematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (Bond^TM Polymer Refine Detection).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on human cerebrum.

The section was incubated with ab178680 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free (AB221933)

This data was developed using ab178680, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Iba1 with purified ab178680 at 1 : 100 dilution (9.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free (AB221933)

This data was developed using ab178680, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Iba1 with purified ab178680 at 1 : 16000 (0.06 μg/ml). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free (AB221933)

This data was developed using ab178680, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling Iba1 with purified ab178680 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free (AB221933)

This data was developed using ab178680, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Iba1 with purified ab178680 at 1 : 16000 (0.06 μg/ml). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free (AB221933)

This data was developed using ab178680, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Paraffin-embedded sections Human tonsil tissue labelling Iba1 with ab178680 at 1/100000 dilution, followed by a ready to use secondary LeicaDS9800 (Bond^TM Polymer Refine Detection). Counter stained with Haematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (Bond^TM Polymer Refine Detection).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on human tonsil.

The section was incubated with ab178680 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free (AB221933)

This data was developed using ab178680, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Paraffin-embedded sections Human glioma tissue labelling Iba1 with ab178680 at 1/100000 dilution, followed by a ready to use secondary LeicaDS9800 (Bond^TM Polymer Refine Detection). Counter stained with Haematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (Bond^TM Polymer Refine Detection).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on human glioma.

The section was incubated with ab178680 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Supplier Data

Western blot - Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free (AB221933)

This data was developed using ab178680, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (<a href='/en-us/products/primary-antibodies/iba1-antibody-epr61362-microglia-marker-ab178680'>ab178680</a>) at 1/1000 dilution

All lanes:

THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 16 kDa

Observed band size: 17 kDa

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