Anti-Iba1 antibody - Microglia marker

• IHC-P

AbReview36799****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody - Microglia marker (AB107159)

ab107159 staining Iba1 in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted HRP-conjugated anti-goat IgG polyclonal was used as the secondary antibody. Kupffer cells are positive for Iba-1.

This image is courtesy of an anonymous Abreview

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody - Microglia marker (AB107159)

IHC image of Iba1 staining in a section of formalin-fixed paraffin-embedded normal rat brain performed on a Leica BOND^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution

1. for 20mins. The section was then incubated with ab107159, 5ug/ml, for 15 mins at room temperature. An HRP-conjugated donkey anti-goat IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

AbReview31800****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody - Microglia marker (AB107159)

IHC-P image of Iba1 staining on rat spleen sections using ab107159 (1 : 2000). The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours. The secondary antibody was goat polyclonal to anti-sheep conjugated to biotin at 1 : 250.

This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

• IHC-P

AbReview33725****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody - Microglia marker (AB107159)

IHC-P image of Iba1 staining on marmoset (common) kidney sections using ab107159 (1 : 5000). The image shows macrophage infiltration in the cortex. The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 16 hours. The secondary antibody was rabbit polyclonal to sheep IgG conjugated to biotin at 1 : 200.

This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom