Mouse Recombinant Monoclonal Iba1 antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human AIF1 aa 1 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [rAIF1/1909] - BSA and Azide free (AB237830), expandable thumbnail](https://content.abcam.com/products/images/iba1-antibody-raif1-1909-bsa-and-azide-free-ab237830--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129854.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [rAIF1/1909] - BSA and Azide free (AB237830)")
![Protein Array - Anti-Iba1 antibody [rAIF1/1909] - BSA and Azide free (AB237830), expandable thumbnail](https://content.abcam.com/products/images/iba1-antibody-raif1-1909-bsa-and-azide-free-ab237830--protein-array-img145310.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Iba1 antibody [rAIF1/1909] - BSA and Azide free (AB237830)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human AIF1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P55008
Reactivity data
Select an application| Protein Array | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Recombinant full length protein - Human | Tested | Not recommended |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 mins at RT. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Alternative names
G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1
Recommended products
Mouse Recombinant Monoclonal Iba1 antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human AIF1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human AIF1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P55008
- Carrier free
- Yes
- Clone number
- rAIF1/1909
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab237830 is the carrier-free version of Anti-Iba1 antibody [rAIF1/1909] ab237988.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.
- Biological function summary
Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.
- Pathways
Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.
- Associated diseases and disorders
Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [rAIF1/1909] - BSA and Azide free (ab237830), expandable thumbnail](https://content.abcam.com/products/images/iba1-antibody-raif1-1909-bsa-and-azide-free-ab237830--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129854.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [rAIF1/1909] - BSA and Azide free (ab237830)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [rAIF1/1909] - BSA and Azide free (ab237830)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Iba1 using Anti-Iba1 antibody [rAIF1/1909] ab237988 at 2 μg/mL in immunohistochemical analysis.
This data was produced with Anti-Iba1 antibody [rAIF1/1909] ab237988, the same antibody in a different formulation with BSA and Azide.
![Protein Array - Anti-Iba1 antibody [rAIF1/1909] - BSA and Azide free (ab237830), expandable thumbnail](https://content.abcam.com/products/images/iba1-antibody-raif1-1909-bsa-and-azide-free-ab237830--protein-array-img145310.jpg "Protein Array - Anti-Iba1 antibody [rAIF1/1909] - BSA and Azide free (ab237830)")
Protein Array - Anti-Iba1 antibody [rAIF1/1909] - BSA and Azide free (ab237830)
This data was produced with Anti-Iba1 antibody [rAIF1/1909] ab237988, the same antibody in a different formulation with BSA and Azide.
Anti-Iba1 antibody [rAIF1/1909] ab237988 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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