Anti-ICAD antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody (AB264202)

Immunohistochemical analysis of formalin/PFA-fixed, paraffin-embedded human ovarian tissue sections labeling ICAD with ab264202 at 1/200 dilution. Detection : DAB.

• IP

Supplier Data

Immunoprecipitation - Anti-ICAD antibody (AB264202)

ICAD was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264202 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264202 at 1 μg/ml.
Lane 1 : ab264202 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 1 sec.

All lanes:

Immunoprecipitation - Anti-ICAD antibody (ab264202)

Predicted band size: 37 kDa

false

• WB

Supplier Data

Western blot - Anti-ICAD antibody (AB264202)

Detected with chemilimuniscence with exposure time of 30 seconds.

4-20% SDS-PAGE.

Secondary : Goat anti-Rabbit Light Chain HRP Conjugate.

All lanes:

Western blot - Anti-ICAD antibody (ab264202) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T whole cell lysate at 50 µg

Predicted band size: 37 kDa

false