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AB108924

Anti-ICAD antibody [EPR4220]

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(2 Publications)

Rabbit Recombinant Monoclonal ICAD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

DFF1, DFF45, H13, DFFA, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, Inhibitor of CAD, DFF-45, ICAD

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] (AB108924)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] (AB108924)

Immunohistochemical staining of paraffin-embedded human breast carcinoma using ab108924 at a diltion of 1/500.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] (AB108924)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] (AB108924)

Immunofluorescent staining of HeLa cells using ab108924 at a dilution of 1/250.

Western blot - Anti-ICAD antibody [EPR4220] (AB108924)
  • WB

Unknown

Western blot - Anti-ICAD antibody [EPR4220] (AB108924)

All lanes:

Western blot - Anti-ICAD antibody [EPR4220] (ab108924) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg/mL

Lane 2:

HeLa cell lysate at 10 µg/mL

Predicted band size: 37 kDa

false

Western blot - Anti-ICAD antibody [EPR4220] (AB108924)
  • WB

Lab

Western blot - Anti-ICAD antibody [EPR4220] (AB108924)

Western blot : Anti-ICAD antibody [EPR4220] ab108924 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type A549 cell lysates with no signal observed at this size in DFFA CRISPR-Cas9 Edited A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ICAD antibody [EPR4220] (ab108924) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

DFFA CRISPR-Cas9 Edited A549 at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

THP-1 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 40 kDa,50 kDa

false

  • Carrier free

    Anti-ICAD antibody [EPR4220] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4220

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitor of the caspase-activated DNase (DFF40).
See full target information DFFA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 20:2 PubMed36609216

2023

Investigation on the mechanisms of human sperm DNA damage based on the proteomics analysis by SWATH-MS.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Hui Zhu,Ye Wei,Fang Chen,Feng Li,Sheng-Min Zhang,Nai-Jun Dong,Tong-Min Xue,Kai-Feng Liu,Heng-Mi Cui,Jin-Chun Lu

Neurourology and urodynamics 36:2011-2018 PubMed28346707

2017

Periurethral muscle-derived mononuclear cell injection improves urethral sphincter restoration in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Marcelo Pitelli Turco,Alex Balduino de Souza,Isida de Campos Sousa,Paula Fratini,Mariana Matera Veras,Marcio Nogueira Rodrigues,José de Bessa,Marina Pandolphi Brolio,Katia Ramos Moreira Leite,Homero Bruschini,Miguel Srougi,Maria Angélica Miglino,Cristiano Mendes Gomes
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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