Rabbit Recombinant Monoclonal ICAD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-ICAD antibody [EPR4220] (AB108924), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-ab108924--western-blot-img110920.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ICAD antibody [EPR4220] (AB108924)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] (AB108924), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-ab108924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92676.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] (AB108924)")
![Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] (AB108924), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-ab108924--immunocytochemistry-immunofluorescence-img18066.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] (AB108924)")
Publications
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- Clinical proteomics 20:22023Investigation on the mechanisms of human sperm DNA damage based on the proteomics analysis by SWATH-MS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Hui Zhu,Ye Wei,Fang Chen,Feng Li,Sheng-Min Zhang,Nai-Jun Dong,Tong-Min Xue,Kai-Feng Liu,Heng-Mi Cui,Jin-Chun LuPubMed 36609216
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Inhibitor of the caspase-activated DNase (DFF40).
Alternative names
DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, Inhibitor of CAD, DFF-45, ICAD, DFF45, H13, DFFA, DFF1
Recommended products
Rabbit Recombinant Monoclonal ICAD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR4220
- Purity
Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-ICAD antibody [EPR4220] (ab108924), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-ab108924--western-blot-img110920.jpg "Western blot - Anti-ICAD antibody [EPR4220] (ab108924)")
Western blot - Anti-ICAD antibody [EPR4220] (ab108924)
All lanes: Western blot - Anti-ICAD antibody [EPR4220] (ab108924) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg/mL
Lane 2: HeLa cell lysate at 10 µg/mL
Predicted band size: 37 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] (ab108924), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-ab108924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92676.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] (ab108924)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] (ab108924)
Immunohistochemical staining of paraffin-embedded human breast carcinoma using ab108924 at a diltion of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] (ab108924), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-ab108924--immunocytochemistry-immunofluorescence-img18066.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] (ab108924)")
Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] (ab108924)
Immunofluorescent staining of HeLa cells using ab108924 at a dilution of 1/250.
Downloads
Product protocols
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