Anti-ICAD antibody [EPR4220] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] - BSA and Azide free (AB247711)

This data was developed using ab108924, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin-embedded human breast carcinoma using ab108924 at a diltion of 1/500.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] - BSA and Azide free (AB247711)

This data was developed using ab108924, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using ab108924 at a dilution of 1/250.

• WB

Unknown

Western blot - Anti-ICAD antibody [EPR4220] - BSA and Azide free (AB247711)

This data was developed using ab108924, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ICAD antibody [EPR4220] (<a href='/en-us/products/primary-antibodies/icad-antibody-epr4220-ab108924'>ab108924</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg/mL

Lane 2:

HeLa cell lysate at 10 µg/mL

Predicted band size: 37 kDa

false

• WB

Lab

Western blot - Anti-ICAD antibody [EPR4220] - BSA and Azide free (AB247711)

This data was developed using ab108924, the same antibody clone in a different buffer formulation.

Western blot : Anti-ICAD antibody [EPR4220] ab108924 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type A549 cell lysates with no signal observed at this size in DFFA CRISPR-Cas9 Edited A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ICAD antibody [EPR4220] (<a href='/en-us/products/primary-antibodies/icad-antibody-epr4220-ab108924'>ab108924</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

DFFA CRISPR-Cas9 Edited A549 at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

THP-1 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 40 kDa,50 kDa

false