Rabbit Recombinant Monoclonal ICAD antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-ICAD antibody [EPR4220] - BSA and Azide free (AB247711), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-bsa-and-azide-free-ab247711--western-blot-img110920.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ICAD antibody [EPR4220] - BSA and Azide free (AB247711)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] - BSA and Azide free (AB247711), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-bsa-and-azide-free-ab247711--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92676.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] - BSA and Azide free (AB247711)")
![Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] - BSA and Azide free (AB247711), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-bsa-and-azide-free-ab247711--immunocytochemistry-immunofluorescence-img18066.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] - BSA and Azide free (AB247711)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Inhibitor of the caspase-activated DNase (DFF40).
Alternative names
DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, Inhibitor of CAD, DFF-45, ICAD, DFF45, H13, DFFA, DFF1
Recommended products
Rabbit Recombinant Monoclonal ICAD antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Alternative names
DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, Inhibitor of CAD, DFF-45, ICAD, DFF45, H13, DFFA, DFF1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR4220
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab247711 is the carrier-free version of Anti-ICAD antibody [EPR4220] ab108924.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-ICAD antibody [EPR4220] - BSA and Azide free (ab247711), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-bsa-and-azide-free-ab247711--western-blot-img110920.jpg "Western blot - Anti-ICAD antibody [EPR4220] - BSA and Azide free (ab247711)")
Western blot - Anti-ICAD antibody [EPR4220] - BSA and Azide free (ab247711)
This data was developed using Anti-ICAD antibody [EPR4220] ab108924, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ICAD antibody [EPR4220] (Anti-ICAD antibody [EPR4220] ab108924) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg/mL
Lane 2: HeLa cell lysate at 10 µg/mL
Predicted band size: 37 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] - BSA and Azide free (ab247711), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-bsa-and-azide-free-ab247711--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92676.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] - BSA and Azide free (ab247711)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody [EPR4220] - BSA and Azide free (ab247711)
This data was developed using Anti-ICAD antibody [EPR4220] ab108924, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin-embedded human breast carcinoma using Anti-ICAD antibody [EPR4220] ab108924 at a diltion of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] - BSA and Azide free (ab247711), expandable thumbnail](https://content.abcam.com/products/images/icad-antibody-epr4220-bsa-and-azide-free-ab247711--immunocytochemistry-immunofluorescence-img18066.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] - BSA and Azide free (ab247711)")
Immunocytochemistry/ Immunofluorescence - Anti-ICAD antibody [EPR4220] - BSA and Azide free (ab247711)
This data was developed using Anti-ICAD antibody [EPR4220] ab108924, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using Anti-ICAD antibody [EPR4220] ab108924 at a dilution of 1/250.
Downloads
Product protocols
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