Anti-ICAD antibody [EPR4221] - BSA and Azide free

• WB

Unknown

Western blot - Anti-ICAD antibody [EPR4221] - BSA and Azide free (AB248425)

This data was developed using ab133250, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ICAD antibody [EPR4221] - BSA and Azide free (ab248425) at 1/10000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

U937 cell lysates at 10 µg

Lane 3:

A431 cell lysates at 10 µg

Lane 4:

HeLa cell lysates at 10 µg

Predicted band size: 37 kDa

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• WB

Lab

Western blot - Anti-ICAD antibody [EPR4221] - BSA and Azide free (AB248425)

This data was developed using ab133250, the same antibody clone in a different buffer formulation.

Western blot : Anti-ICAD antibody [EPR4221] ab133250 staining at 1/10000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 40 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ICAD antibody [EPR4221] (<a href='/en-us/products/primary-antibodies/icad-antibody-epr4221-ab133250'>ab133250</a>) at 1/10000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

DFFA CRISPR-Cas9 Edited A549 at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

THP-1 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 40 kDa,52 kDa

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