AB20

Anti-ICAM1 antibody [15.2]

Name: Anti-ICAM1 antibody [15.2] (ab20) | Abcam
Brand: Abcam Limited
SKU: AB20
Rating: 1 (1 reviews)

(1 Review)

(28 Publications)

Mouse Monoclonal ICAM1 antibody. Suitable for WB and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Cell preparation containing ICAM1 protein.

View Alternative Names

CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1

1 Images

Unknown

Western blot - Anti-ICAM1 antibody [15.2] (AB20)

All lanes:

Western blot - Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/mL

Lane 1:

SK N BE (Human neuroblastoma) whole cell lysate at 10 µg

Lane 2:

Saos-2 (Human epithelial-like osteosarcoma cell line) whole cell lysate at 10 µg

Lane 3:

HT1080 (Human fibrosarcoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 57 kDa

Observed band size: 58 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

15.2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Cell preparation containing ICAM1 protein. The exact immunogen used to generate this antibody is proprietary information.

P05362

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze|Store undiluted

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins.. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.

See full target information ICAM1

style

left-column

Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7262 PubMed40769972

2025

Plasmodium falciparum egress disrupts endothelial junctions and activates JAK-STAT signaling in a microvascular 3D blood-brain barrier model.

Applications

Unspecified application

Species

Unspecified reactive species

Livia Piatti,Alina Batzilla,Fumio Nakaki,Hannah Fleckenstein,François Korbmacher,Rory K M Long,Daniel Schraivogel,John A Hawkins,Tais Romero-Uruñuela,Borja López-Gutiérrez,Silvia Sanz Sender,Yannick Schwab,Lars M Steinmetz,James Sharpe,Maria Bernabeu

Experimental and therapeutic medicine 25:240 PubMed37114177

2023

Tyrosol attenuates lipopolysaccharide‑induced inflammation in HUVECs to promote vascular health against atherosclerosis challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Zhao,Huizhen Wei,Jun Lu,Wenjun Sha,Dusang Sun,Ting Pan,Tao Lei

Frontiers in cell and developmental biology 8:15 PubMed32083076

2020

Agrin Influences Botulinum Neurotoxin A-Induced Nerve Sprouting via miR-144-agrin-MuSK Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Ma,Lizhen Pan,Wuchao Liu,Ying Liu,Xuerui Xiang,Yougui Pan,Xiaolong Zhang,Lingjing Jin

Nature communications 10:5688 PubMed31831736

2019

Involvement of condensin in cellular senescence through gene regulation and compartmental reorganization.

Applications

Unspecified application

Species

Unspecified reactive species

Osamu Iwasaki,Hideki Tanizawa,Kyoung-Dong Kim,Andrew Kossenkov,Timothy Nacarelli,Sanki Tashiro,Sonali Majumdar,Louise C Showe,Rugang Zhang,Ken-Ichi Noma

Medical science monitor : international medical jo 25:6946-6954 PubMed31860633

2019

The Effects of the CXCR4 Antagonist, AMD3465, on Human Retinal Vascular Endothelial Cells (hRVECs) in a High Glucose Model of Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wu,Li Jin,Hongshuang Xu

PPAR research 2019:2715176 PubMed31354796

2019

Peroxisome Proliferator-Activated Receptor-γ Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Xu,Gang Zhao,Hong Zhu,Shijun Wang,Aijun Sun,Yunzeng Zou,Junbo Ge

mBio 10: PubMed31138740

2019

Binding Heterogeneity of Plasmodium falciparum to Engineered 3D Brain Microvessels Is Mediated by EPCR and ICAM-1.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Bernabeu,Celina Gunnarsson,Maria Vishnyakova,Caitlin C Howard,Ryan J Nagao,Marion Avril,Terrie E Taylor,Karl B Seydel,Ying Zheng,Joseph D Smith

International journal of molecular sciences 20: PubMed30577658

2018

Protective Effects of Total Saponins of (Miq.) on Endothelial Cell Injury Induced by TNF-α via Modulation of the PI3K/Akt and NF-κB Signalling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zhou,Weijie Xie,Yun Luo,Shan Lu,Ziru Dai,Ruiying Wang,Guibo Sun,Xiaobo Sun

Journal of colloid and interface science 538:630-637 PubMed30554096

2018

Intercellular adhesion molecule 1 antibody-mediated mesoporous drug delivery system for targeted treatment of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mengru Wang,Wanhua Liu,Yanqiu Zhang,Meng Dang,Yunlei Zhang,Jun Tao,Kun Chen,Xin Peng,Zhaogang Teng

Frontiers in immunology 9:331 PubMed29535719

2018

Tissue Damage Caused by Myeloablative, but Not Non-Myeloablative, Conditioning before Allogeneic Stem Cell Transplantation Results in Dermal Macrophage Recruitment without Active T-Cell Interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Peter van Balen,Boris van der Zouwen,Alwine B Kruisselbrink,Matthijs Eefting,Karoly Szuhai,Ekaterina S Jordanova,J H F Falkenburg,Inge Jedema

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB53013

Anti-ICAM1 antibody [EP1442Y]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (AB53013)

Primary Antibodies

AB179707

Anti-ICAM1 antibody [EPR16608]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (AB179707)

Primary Antibodies

AB221777

Anti-ICAM1 antibody [EP1442Y] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] - Low endotoxin, Azide free (AB221777)

Primary Antibodies

AB109361

Anti-ICAM1 antibody [EPR4776]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR4776] (AB109361)

Primary Antibodies

AB222736

Anti-ICAM1 antibody [EPR22161-284]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR22161-284] (AB222736)

Primary Antibodies

AB206398

Anti-ICAM1 antibody [EPR19938]

RabMAb|Recombinant

Western blot - Anti-ICAM1 antibody [EPR19938] (AB206398)

Complementary Products

Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com