Anti-ICAM1 antibody [EPR19938] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (AB251453)

This data was developed using ab206398, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ICAM1 with ab206398 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membranous staining on sinusoidal endothelial cells of rat liver [PMID : 17058232].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (AB251453)

This data was developed using ab206398, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ICAM1 with ab206398 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Positive staining on extracellular matrix and blood vessels of rat kidney [PMID : 9198039] [PMID 7902311].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (AB251453)

This data was developed using ab206398, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-4 : 5 seconds; Lanes 5-6 : 1 minute.

The observed molecular weight is consistent with the literature (PMID : 1712683).

All lanes:

Western blot - Anti-ICAM1 antibody [EPR19938] (<a href='/en-us/products/primary-antibodies/icam1-antibody-epr19938-ab206398'>ab206398</a>) at 1/5000 dilution

Lane 1:

Rat spleen lysate at 20 µg

Lane 2:

Rat lung lysate at 20 µg

Lane 3:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 4:

L6 (Rat skeletal muscle cell line) whole cell lysate at 10 µg

Lane 5:

Rat liver lysate at 20 µg

Lane 6:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 80-110 kDa

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