Anti-ICAM1 antibody [EPR24639-3] – Mouse IgG1 (Chimeric) – BSA and Azide Free
- KO Validated
- Recombinant
- What is this?
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Mouse Recombinant Monoclonal ICAM1 antibody. Carrier free. Suitable for ICC/IF and reacts with Human samples.
View Alternative Names
CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1
![Immunocytochemistry/ Immunofluorescence - Anti-ICAM1 antibody [EPR24639-3] – Mouse IgG1 (Chimeric) – BSA and Azide Free (AB323253)](https://content.abcam.com/products/images/icam1-antibody-epr24639-3-mouse-igg1-chimeric-bsa-and-azide-free-ab323253--immunocytochemistry-immunofluorescence-img440853.jpg/_jcr_content/renditions/webp-768.webp)
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-ICAM1 antibody [EPR24639-3] – Mouse IgG1 (Chimeric) – BSA and Azide Free (AB323253)
ab323244 staining ICAM1 in HeLa (positive) and HeLa-ICAM1 KO (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4° C with ab323244 at 1ug/ml (shown in green) and ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
This data was developed using the same antibody clone in a different buffer formulation.
Related conjugates and formulations (1)
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Anti-ICAM1 antibody [EPR24639-3] – Mouse IgG1 (Chimeric)
Reactivity data
Product details
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab282575). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
ab323253 is the carrier-free version of ab323244
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Product protocols
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Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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