Mouse Monoclonal ICAM1 antibody. Suitable for WB, Flow Cyt and reacts with Human samples. Cited in 77 publications. Immunogen corresponding to Cell preparation containing ICAM1 protein.
Images
![Western blot - Anti-ICAM1 antibody [MEM-111] (AB2213), expandable thumbnail](https://content.abcam.com/products/images/icam1-antibody-mem-111-ab2213--western-blot-img134353.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ICAM1 antibody [MEM-111] (AB2213)")
![Western blot - Anti-ICAM1 antibody [MEM-111] (AB2213), expandable thumbnail](https://content.abcam.com/products/images/icam1-antibody-mem-111-ab2213--western-blot-img422657.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ICAM1 antibody [MEM-111] (AB2213)")
![Flow Cytometry - Anti-ICAM1 antibody [MEM-111] (AB2213), expandable thumbnail](https://content.abcam.com/products/images/icam1-antibody-mem-111-ab2213--flow-cytometry-img422658.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-ICAM1 antibody [MEM-111] (AB2213)")
Publications
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- Fluids and barriers of the CNS 20:802023A tissue-engineered model of the blood-tumor barrier during metastatic breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaleigh M Linville,Joanna Maressa,Zhaobin Guo,Tracy D Chung,Alanna Farrell,Ria Jha,Peter C SearsonPubMed 37924145
- Frontiers in microbiology 14:11069452023Altered cell function and increased replication of rhinoviruses and EV-D68 in airway epithelia of asthma patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManel Essaidi-Laziosi,Léna Royston,Bernadett Boda,Francisco Javier Pérez-Rodriguez,Isabelle Piuz,Nicolas Hulo,Laurent Kaiser,Sophie Clément,Song Huang,Samuel Constant,Caroline TapparelPubMed 36937308
- Viruses 14:2022Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWouter Johannes Petrus van den Braak,Bella Monica,Diana Limpens,Dedeke Rockx-Brouwer,Matthijn de Boer,Dinja OosterhoffPubMed 36298792
- Journal of molecular and cellular cardiology 170:75-862022ICAM-1-related noncoding RNA accelerates atherosclerosis by amplifying NF-κB signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuangjin Ding,Jiankun Liu,XiaoRui Han,Wanqiu Ding,Zhirui Liu,Ying Zhu,Wenxing Zhan,Yiqi Wan,Shujie Gai,Junjie Hou,Xiaoxia Wang,Yixia Wu,Andong Wu,Chuan-Yun Li,Zhe Zheng,Xiao-Li Tian,Huiqing CaoPubMed 35714558
- Lab on a chip 22:1929-19422022Subtractive manufacturing with swelling induced stochastic folding of sacrificial materials for fabricating complex perfusable tissues in multi-well plates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShravanthi Rajasekar,Dawn S Y Lin,Feng Zhang,Alexander Sotra,Alex Boshart,Sergi Clotet-Freixas,Amy Liu,Jeremy A Hirota,Shinichiro Ogawa,Ana Konvalinka,Boyang ZhangPubMed 35383790
- Cell death & disease 13:4172022ICAM-1 promotes cancer progression by regulating SRC activity as an adapter protein in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Ji Lim,Jae-Hyeok Kang,Yeon-Ju Kim,Seungmo Kim,Su-Jae LeePubMed 35487888
- Medicina (Kaunas, Lithuania) 58:2022Impact of Activation of within Microenvironment of High Grade Ovarian Serous Carcinoma on Infiltration of CD4+ and CD8+ Lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacek J Sznurkowski,Anton Żawrocki,Natalia Krawczyńska,Michał Bieńkowski,Bartosz Wasąg,Wojciech BiernatPubMed 35630004
- Frontiers in oncology 12:8017642022Platinum Chemotherapy Induces Lymphangiogenesis in Cancerous and Healthy Tissues That Can be Prevented With Adjuvant Anti-VEGFR3 Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra R Harris,Savieay Esparza,Mohammad S Azimi,Robert Cornelison,Francesca N Azar,Danielle C Llaneza,Maura Belanger,Alexander Mathew,Svyatoslav Tkachenko,Matthew J Perez,Claire Buchta Rosean,Raegan R Bostic,R Chase Cornelison,Kinsley M Tate,Shayn M Peirce-Cottler,Cherie Paquette,Anne Mills,Charles N Landen,Jeff Saucerman,Patrick M Dillon,Rebecca R Pompano,Melanie A Rutkowski,Jennifer M MunsonPubMed 35372032
- medRxiv : the preprint server for health sciences :2022Endothelial cell-activating antibodies in COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Shi,Yu Zuo,Sherwin Navaz,Alyssa Harbaugh,Claire K Hoy,Alex A Gandhi,Gautam Sule,Srilakshmi Yalavarthi,Kelsey Gockman,Jacqueline A Madison,Jintao Wang,Melanie Zuo,Yue Shi,Michael D Maile,Jason S Knight,Yogendra KanthiPubMed 33501469
- Pulmonary circulation 11:204589402110568062021Evidence supporting a role for circulating macrophages in the regression of vascular remodeling following sub-chronic exposure to hemoglobin plus hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVijaya Karoor,Delaney Swindle,David I Pak,Derek Strassheim,Mehdi A Fini,Edward Dempsey,Kurt R Stenmark,Kathryn Hassell,Rachelle Nuss,Paul W Buehler,David C IrwinPubMed 34777787
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing ICAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05362
Abcam Recommends
Reactivity data
Select an application| WB | Flow Cyt | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Use under non-reducing conditions. |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-4.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Alternative names
CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1
Recommended products
Mouse Monoclonal ICAM1 antibody. Suitable for WB, Flow Cyt and reacts with Human samples. Cited in 77 publications. Immunogen corresponding to Cell preparation containing ICAM1 protein.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing ICAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05362
- Clone number
- MEM-111
- Purification technique
- Affinity purification Protein A
- Specificity
his antibody reacts with human CD54 antigen.
- Light chain type
- unknown
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213), expandable thumbnail](https://content.abcam.com/products/images/icam1-antibody-mem-111-ab2213--western-blot-img134353.jpg "Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213)")
Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213)
Lower bands represent tubulin as a loading control.
All lanes: Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213)
Lane 1: TNF-alpha activated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 2: TNF-alpha nonactivated HUVEC cells
Predicted band size: 57 kDa
Observed band size: 90 kDa
![Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213), expandable thumbnail](https://content.abcam.com/products/images/icam1-antibody-mem-111-ab2213--western-blot-img422657.jpg "Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213)")
Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213)
All lanes: Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213) at 2 µg/mL
Lane 1: TF-1 cell lysates under non-reducing condition
Lane 2: Raji cell lysates under non-reducing condition
Lane 3: Jurkat cell lysates under non-reducing condition
Lane 4: TF-1 cell lysates under reducing condition
Lane 5: Raji cell lysates under reducing condition
Lane 6: Jurkat cell lysates under reducing condition
SecondaryAll lanes: IRDye800-conjugated anti-mouse secondary antibody
Predicted band size: 57 kDa
Observed band size: 88 kDa
![Flow Cytometry - Anti-ICAM1 antibody [MEM-111] (ab2213), expandable thumbnail](https://content.abcam.com/products/images/icam1-antibody-mem-111-ab2213--flow-cytometry-img422658.jpg "Flow Cytometry - Anti-ICAM1 antibody [MEM-111] (ab2213)")
Flow Cytometry - Anti-ICAM1 antibody [MEM-111] (ab2213)
Flow cytometric analysis of Human Peripheral Blood cells labelling ICAM1 with ab2213 at 0.6 ug/ml showing separation of human monocytes (red-filled) from human lymphocytes (black-dashed).
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