Rabbit Polyclonal ICAM2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Icam2 aa 1-250.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Icam2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P35330
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.20000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.20000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
Alternative names
Intercellular adhesion molecule 2, ICAM-2, ICAM2
Recommended products
Rabbit Polyclonal ICAM2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Icam2 aa 1-250.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Icam2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P35330
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab231564 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ICAM2 also known as IC2 or ICAM-2 is a cell surface glycoprotein with a molecular mass of approximately 55-65 kDa. It plays an important role in immune response. The protein belongs to the immunoglobulin superfamily and interacts specifically with integrin molecules. ICAM2 is expressed widely across many tissues including endothelial cells platelets and leukocytes. Its primary function involves facilitating stable adhesion between leukocytes and endothelial cells which is essential for leukocyte transmigration.
- Biological function summary
ICAM2 helps in immune function by serving as an adhesion molecule. It does not form part of a larger protein complex but instead it acts independently to mediate interactions between cells. It contributes to leukocyte rolling and firm adhesion stages necessary for leukocyte extravasation. This function is important in immune surveillance and response to infection allowing immune cells to quickly migrate to sites of inflammation.
- Pathways
ICAM2 operates in the leukocyte adhesion and transendothelial migration pathways. It interacts closely with integrins especially LFA-1 present on leukocytes and this interaction supports cell movement during immune responses. It also participates in signaling pathways that activate immune cells further aiding in the regulation of immune cell activity. These interactions make ICAM2 a critical component in immunological communication and signal transduction pathways.
- Associated diseases and disorders
ICAM2 is associated with conditions involving immune dysfunction and inflammation. For instance abnormal expression of ICAM2 has been linked to inflammatory diseases such as atherosclerosis. Additionally it plays a role in the pathology of multiple sclerosis where it might influence the migration of immune cells across the blood-brain barrier. Its relationship with integrins highlights its potential relevance in therapeutic strategies to modulate immune responses in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-ICAM2 antibody (ab231564)
All lanes: Western blot - Anti-ICAM2 antibody (ab231564) at 2 µg/mL
All lanes: Recombinant mouse ICAM2 protein
Predicted band size: 31 kDa
Western blot - Anti-ICAM2 antibody (ab231564)
All lanes: Western blot - Anti-ICAM2 antibody (ab231564) at 2 µg/mL
All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody (ab231564)
Formalin-fixed, paraffin-embedded mouse spleen tissue stained for ICAM2 using ab231564 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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